|
| Status |
Public on May 01, 2011 |
| Title |
Alcohol Consumption Effects During the Inflammatory Stage of Fracture Healing in Rat Bones |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Sprague-Dawley rats were placed on an ethanol-containing or pair-fed Lieber and DeCarli diets for 4 wks prior to surgical fracture. Following insertion of a medullary pin, a closed mid-diaphyseal fracture was induced using a Bonnarens and Einhorn fracture device. At 3 days post-fracture, the region of the fracture calluses were harvested from the right hind-limb. RNA was extracted and microarray analysis was conducted against the entire rat genome to study the effects of alcohol-consumption on the fracture healing.
|
| |
|
| Overall design |
The experiments were on four rat subjects, i.e., pair-fed rats with subsequent surgical fracture or no surgical fracture, and alcohol-fed rats with subsequent surgical fracture or no surgical fracture. Each rat subject described above has three replicates so 6 kinds of pairing can be made and each pairing has a dye-swap replicate (thus, a total 12 array experiments). The focus of this study is on the pair-fed fracture subject vs. alcohol-fed fracture subject.
|
| |
|
| Contributor(s) |
Sampson HW, Huang H, VanBuren VJ |
| Citation(s) |
22087020 |
| |
| Submission date |
May 06, 2009 |
| Last update date |
Jan 18, 2013 |
| Contact name |
H Wayne Sampson |
| E-mail(s) |
sampson@medicine.tamhsc.edu
|
| Phone |
254-231-5340
|
| Fax |
254-742-7145
|
| Organization name |
TAMHSC-College of Medicine
|
| Department |
Systems Biology & Translational Medicine
|
| Street address |
702 SW HK Dodgen Loop
|
| City |
Temple |
| State/province |
TX |
| ZIP/Postal code |
76504 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7471 |
Operon Rat V3.0 printed oligonucleotide array |
|
| Samples (6)
|
| GSM400530 |
A (pair-fed, fracture) #1 vs. B (pair-fed, no fracture) #1 |
| GSM400531 |
A (pair-fed, fracture) #2 vs. C (alcohol-fed, fracture) #1 |
| GSM400532 |
A (pair-fed, fracture) #3 vs. D (alcohol-fed, no fracture) #1 |
| GSM400533 |
B (pair-fed, no fracture) #2 vs. C (alcohol-fed, fracture) #2 |
| GSM400534 |
B (pair-fed, no fracture) #3 vs. D (alcohol-fed, no fracture) #2 |
| GSM400535 |
C (alcohol-fed, fracture) #3 vs. D (alcohol-fed, no fracture) #3 |
|
| Relations |
| BioProject |
PRJNA115421 |
Less...
More...