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| Status |
Public on Apr 01, 2021 |
| Title |
Dynamic post-transcriptional regulation by Mrn1 links cell wall homeostasis to mitochondrial structure and function |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The RNA-binding protein Mrn1 in Saccharomyces cerevisiae targets over 300 messenger RNAs, including many involved in cell wall biogenesis. The impact of Mrn1 on these target transcripts is not known, however, nor is the cellular role for this regulation. We have shown that Mrn1 represses target mRNAs through the action of its disordered, asparagine-rich amino-terminus. Its endogenous targets include the paralogous SUN domain proteins Nca3 and Uth1, which affect mitochondrial structure and function as well as the cell wall, as well as other nuclear-encoded mitochondrial proteins. While loss of MRN1 has no effect on fermentative growth, we found that mrn1Δ yeast adapt more quickly to respiratory conditions. These cells also have enlarged mitochondria in fermentative conditions, an observation that may explain their faster switch to respiration. Our analyses indicated that Mrn1 acts as a hub for integrating cell wall integrity and mitochondrial biosynthesis in a carbon-source responsive manner.
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| Overall design |
mRNA profiling in wild-type and mrn1Δ budding yeast, during fermentative (glucose) and respiratory (ethanol + glycerol) growth
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| Contributor(s) |
Reynaud KK, Brothers M, Ly M, Ingolia NT |
| Citation(s) |
33857138 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| DP2 CA195768 |
Molecular Basis and Cellular Roles of Translational Regulation |
UNIVERSITY OF CALIFORNIA BERKELEY |
NICHOLAS T INGOLIA |
| R01 GM130996 |
Functional genomics of the dynamic molecular network controlling mRNA translation and decay |
UNIVERSITY OF CALIFORNIA BERKELEY |
NICHOLAS T INGOLIA |
| S10 OD018174 |
Pacific Biosciences PacBio RS Single Molecule Real Time Sequencer |
UNIVERSITY OF CALIFORNIA BERKELEY |
Daniel Soleyman Rokhsar |
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| |
| Submission date |
Oct 26, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Nicholas T Ingolia |
| E-mail(s) |
ingolia@berkeley.edu, nick@ingolia.org
|
| Phone |
510 664 7071
|
| Organization name |
University of California, Berkeley
|
| Department |
Molecular and Cell Biology
|
| Lab |
Ingolia
|
| Street address |
1 Barker Hall # 3202
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA672166 |
| SRA |
SRP288914 |
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