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| Status |
Public on Feb 17, 2021 |
| Title |
Integrated proteomic and transcriptomic analyses reveal the roles of Brucella BI-1 in cell division and membrane homeostasis of Brucella suis S2 |
| Organism |
Brucella suis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the role of Brucella BI-1 in Brucella suis S2, we constructed the Brucella BI-1 deletion mutant strain and its complementary strain. We then determined the effect of Brucella BI-1 deletion on the physiological characteristics of Brucella suis S2 and revealed them via integrated transcriptomic and proteomic analyses. Brucella BI-1 deletion altered the membrane properties of Brucella suis S2 and decreased its resistance to acidic pH, H2O2, polymyxin B, and lincomycin. Additionally, deleting Brucella BI-1 led to defective growth, cell division, and viability in Brucella suis S2. In conclusion, our results revealed that Brucella BI-1 is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in Brucella suis S2.
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| Overall design |
Transcriptomic profiles of the B. suis S2 WT, ΔBI-1, and cBI-1 strains were generated by deep sequencing, in triplicate.
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| Contributor(s) |
Zhang G, Wang A, Jin Y |
| Citation(s) |
33584633 |
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| Submission date |
Nov 05, 2020 |
| Last update date |
Feb 17, 2021 |
| Contact name |
Guangdong Zhang |
| E-mail(s) |
gdzhang@nwafu.edu.cn
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| Organization name |
Northwest A&F University
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| Department |
College of Veterinary Medicine
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| Street address |
No.22 Xinong Road, Yangling, Shanxi, China
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| City |
Yangling, Shanxi |
| ZIP/Postal code |
712100 |
| Country |
China |
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| Platforms (1) |
| GPL29368 |
Illumina NovaSeq 6000 (Brucella suis) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA674828 |
| SRA |
SRP291310 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160911_genes.count.txt.gz |
55.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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