|
| Status |
Public on Aug 30, 2022 |
| Title |
N-terminus of Drosophila MSL1 interacts with DNA-binding proteins and is critical for the recruitment of the dosage compensation complex to the X chromosome |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The male-specific lethal dosage compensation complex (MSL complex or DCC), which consists of five proteins and two non-coding roX RNAs, is necessary for the transcriptional enhancement of X-linked genes to compensate for the sex chromosome monosomy in Drosophila XY males, compared with XX females. MSL2 is a single protein component of the DCC that is expressed only in males and is essential for the specific recruitment of the DCC to the high-affinity “entry” sites (HASs) on the X chromosome. MSL2, together with MSL1, forms the heterotetrameric DCC core. Here, we demonstrated that the N-terminal unstructured region of MSL1 interacts with many different DNA-binding proteins that contain clusters of the C2H2 zinc-finger domains. Amino acid deletions in the N-terminal region of MSL1 strongly affect the binding of the DCC to the HASs on the male X chromosome. However, the binding of MSL2 to autosomal promoters was unaffected by amino acid deletions in MSL1. Males expressing mutant variants of MSL1 died during the larvae stage, demonstrating the critical role played by the N-terminal region in DCC activity. Our results suggest that MSL1 interacts with a variety of DNA-binding proteins to increase the specificity of DCC recruitment to the male X chromosome.
|
| |
|
| Overall design |
ChIP-seq signal of the MSL1, MSL2 and MSL3 proteins occupancy in adult males and females represented as wt, M1[wt], M1[wt]_M2, M1[Δ41-85]_M2 and M1[Δ1-15]_M2 lines.
|
| |
|
| Contributor(s) |
Maksimenko O, Klimenko N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 09, 2020 |
| Last update date |
Sep 01, 2022 |
| Contact name |
Natalia Klimenko |
| E-mail(s) |
lklimenko@genebiology.ru
|
| Phone |
9150884603
|
| Organization name |
Institute of Gene Biology (IGB) of the Russian Academy of Sciences
|
| Street address |
34/5 Vavilova Street
|
| City |
Moscow |
| ZIP/Postal code |
143026 |
| Country |
Russia |
| |
|
| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA675621 |
| SRA |
SRP291768 |
Less...
More...