|
| Status |
Public on Jan 20, 2021 |
| Title |
Transcriptome profiles of alternative MED19 LNCaP and control LNCaP cells cultured under androgen deprivation with vehicle or R1881 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the application of ChIP and RNA sequencing to identify the mechanism whereby stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation. We determined the MED19 and AR transcriptomes and cistromes in control and MED19 LNCaP cells. We also examined genome-wide H3K27 acetylation in both the absence and presence of androgens. We found that MED19 overexpression selectively alters AR occupancy, H3K27 acetylation, and gene expression. Under conditions of androgen deprivation, genes regulated by MED19 correspond to genes regulated by ELK1, a transcription factor that binds the AR N-terminus to induce select AR target gene expression and proliferation. This study provides important insight into the mechanisms of prostate cancer cell growth under low androgen, and underscores the importance of the MED19 in this process.
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| |
|
| Overall design |
Examination by RNA seq in contorl LNCaP and MED19 LNCaP cells with and without androgen treatment
|
| |
|
| Contributor(s) |
Weber H, Ruoff R, Garabedian M |
| Citation(s) |
33513133 |
| |
| Submission date |
Nov 10, 2020 |
| Last update date |
Jun 21, 2023 |
| Contact name |
Michael Garabedian |
| E-mail(s) |
michael.garabedian@nyumc.org
|
| Phone |
212 263-7662
|
| Fax |
212 263-8276
|
| URL |
http://microbiology-parasitology.med.nyu.edu/node/122
|
| Organization name |
NYU School of Medicine
|
| Department |
Microbiology
|
| Street address |
550 First Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE161268 |
Stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation |
|
| Relations |
| BioProject |
PRJNA676100 |
| SRA |
SRP292182 |
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