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| Status |
Public on Nov 27, 2020 |
| Title |
Data set from hematopoietic stem cell transcriptome elucidating specific targets of Class 1A PI3K |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To detect the global effects of Class1A PI3K TKO deletion on the HSC transcriptome. Method: Whole BM cells from donor mice TKO: Pik3calox-lox; Pik3cblox-lox; Pik3cd-/-;Mx1-Cre, δ KO: Pik3calox-lox; Pik3cblox-lox;Pik3cd-/- (littermate control) and WT: WT, Mx1-Cre (control) were transplanted into lethally irradiated 6-8 week old B6.SJL mice (N=7 per group). Recipient mice were injected 4 weeks post transplant interperitoneally with pIpC 250μg x2, 48 hours apart to ensure Pik3ca and Pik3cb excision. At 4 weeks post PIPC, BM cells were harvested from femurs, tibiae, ilia and vertebrae by gentle crushing. Donor-derived HSCs (Lin-cKit+Sca1+Flk2-CD48-CD150+) cell populations were sorted into RNA extraction buffer (ARCTURUS PicoPure). cDNA was amplified from total RNA using the Ovation® RNA‑Seq System V2. Sequencing was performed using the NGS platform llumina-HiSeq2500/4000.
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| Overall design |
Examination of transcriptome differences in hematopoietic stem cells between non-competitively transplanted Pik3ca lox/lox;Pik3cblox/lox;Pik3cd-/-;Mx1-Cre (TKO) vs Pik3calox/lox;Pik3cblox/lox;Pik3cd-/- (δKO) littermate controls vs WT;Mx1-Cre (WT) controls all samples are in three biological replicates. Each of the biological replicates was done in 2 technical replicates.
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| Contributor(s) |
Gritsman K, Ames K |
| Citation(s) |
36812307 |
| |
| Submission date |
Nov 12, 2020 |
| Last update date |
Mar 02, 2023 |
| Contact name |
Kristina Ames |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Lab |
Gritsman lab
|
| Street address |
1300 Morris Park Avenue, Chanin 410
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA677976 |
| SRA |
SRP292386 |
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