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| Status |
Public on Nov 15, 2020 |
| Title |
Corneal Nonmyelinating Schwann Cells Illuminated by Single Cell Transcriptomics and Visualized by Protein Biomarkers |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The cornea is the most innervated tissue in the human body. Myelinated axons upon inserting into the peripheral corneal stroma lose their myelin sheaths and continue into the central cornea wrapped by only nonmyelinating corneal SCs (nm-cSCs). This anatomical organization is believed to be important for central vision. Here we employed single-cell RNA sequencing (scRNA-seq), microscopy, and transgenics to characterize these nm-cSCs of the central cornea. Using principal component analysis, uniform manifold approximation and projection, and unsupervised hierarchal cell clustering of scRNA-seq data derived from central corneal cells of male rabbits, we successfully identified several clusters representing different corneal cell types, including a unique cell cluster representing nm-cSCs. To confirm protein expression of cSC genes, we performed cross-species validation, employing corneal whole mount immunostaining with confocal microscopy in mouse corneas. We expect that our results will advance the future study of nm-cSCs in applications of nerve repair, and provide a resource for the study of corneal sensory function.
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| Overall design |
Examination of non-myelinating Coreneal Schwann Cells through single cell RNA sequencing
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| Contributor(s) |
Mohan R, Bargagna-Mohan P, Schultz G, Rheaume B, Trakhtenberg E, Robson P, Pal-Ghosh S, Stepp M, Given KS, Macklin WB |
| Citation(s) |
33197966 |
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| Submission date |
Nov 14, 2020 |
| Last update date |
Feb 16, 2021 |
| Contact name |
Royce Mohan |
| E-mail(s) |
mohan@uchc.edu
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| Phone |
860 679 2020
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| Organization name |
University of Connecticut Health Center
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| Department |
Neuroscience
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| Lab |
L4023
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| Street address |
263 Farmington Avenue
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| City |
Farmington |
| State/province |
Connecticut |
| ZIP/Postal code |
06030-3401 |
| Country |
USA |
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| Platforms (1) |
| GPL26786 |
Illumina NovaSeq 6000 (Oryctolagus cuniculus) |
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| Samples (1) |
| GSM4908644 |
Non-myelinating Corneal Schwann Cells scRNA-seq |
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| Relations |
| BioProject |
PRJNA678440 |
| SRA |
SRP292577 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE161487_RAW.tar |
290.0 Kb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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