|
| Status |
Public on Apr 15, 2021 |
| Title |
Regulation of FOXA1 protein stability by Polycomb and BUB3/USP7 deubiquitin complexes in prostate cancer [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
FOXA1 transcription factor is essential for the development and maturation of prostate luminal epithelial cells. FOXA1 gene is frequently mutated and its expression is deregulated during prostate cancer progression. We and others have shown that FOXA1 induces androgen-dependent cell growth by transcriptional regulation of target genes related to cell cycle process, DNA replication, cell division, and apoptosis. Despite of its important roles, how FOXA1 expression itself is regulated in prostate cancer cells remains largely unknown. Here we report that EZH2 methylates one of the lysine residues (K295) on FOXA1 protein to reduce its ubiquitination and subsequent degradation. Mechanistically, this EZH2-mediated FOXA1 methylation recruits BUB3/USP7 deubiquitinase complex to remove FOXA1 ubiquitination for increased protein stability.
|
| |
|
| Overall design |
ChIP-seq of prostate cancer cells with FOXA1 de-regulation or inhibition
|
| |
|
| Contributor(s) |
Yu J, Park KS |
| Citation(s) |
33827814 |
| |
| Submission date |
Nov 16, 2020 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
|
| Organization name |
Emory University
|
| Department |
Urology
|
| Lab |
Jindan Yu's lab
|
| Street address |
E330, 1760 Haygood Dr NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
|
| Samples (12)
|
| GSM4909111 |
HA ChIP-seq in shFoxA1+K295A LNCaP cells |
| GSM4909112 |
FOXA1 ChIP-seq in DMSO treated LNCaP cells_rep #1 |
| GSM4909113 |
FOXA1 ChIP-seq in EPZ-6438 treated LNCaP cells_rep #1 |
| GSM4909114 |
FOXA1 ChIP-seq in P5091 treated LNCaP cells_rep #1 |
| GSM4909115 |
FOXA1 ChIP-seq in EPZ-6438 & P5091 Combo LNCaP cells_rep #1 |
| GSM4909116 |
FOXA1 ChIP-seq in DMSO treated LNCaP cells_ rep #2 |
| GSM4909117 |
FOXA1 ChIP-seq in EPZ-6438 treated LNCaP cells_rep #2 |
| GSM4909118 |
FOXA1 ChIP-seq in P5091 treated LNCaP cells_rep #2 |
| GSM4909119 |
FOXA1 ChIP-seq in EPZ-6438 & P5091 Combo LNCaP cells_rep #2 |
|
| This SubSeries is part of SuperSeries: |
| GSE161519 |
Regulation of FOXA1 protein stability by Polycomb and BUB3/USP7 deubiquitin complexes in prostate cancer |
|
| Relations |
| BioProject |
PRJNA678635 |
| SRA |
SRP292688 |
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