|
| Status |
Public on May 20, 2009 |
| Title |
Differential expression of whole transcriptome in Synechocystis sp. PCC 6803 |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
The model cyanobacterium Synechocystis sp. PCC 6803 was used for a systematic survey of differential expression with a focus on antisense (as)RNAs and non-coding (nc)RNAs. A microarray was constucted with on average 5 probes for each transcript known thus far, including ncRNAs and asRNAs. The resulting 20,431 individual probes are duplicated on the array (Agilent 4x44k custom array) representing a technical replicate.
Hybridization of this array with total RNA isolated from cultures raised under different growth conditions identified transcripts from intergenic spacers and in antisense orientation to known genes (natural cis-asRNAs) with differential expression compared to control hybridizations. This shows the involvement of such transcripts in the regulation of adaptation to various stresses.
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| |
|
| Overall design |
12 RNA hybridizations (1 control & 3 stress conditions, 3 times each)
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| |
|
| Contributor(s) |
Georg J, Voss B, Scholz I, Mitschke J, Wilde A, Hess W |
| Citation(s) |
21245330, 19756044 |
| |
| Submission date |
May 19, 2009 |
| Last update date |
Sep 12, 2014 |
| Contact name |
Bjoern Voss |
| Organization name |
University of Freiburg
|
| Department |
Institute of Biology III
|
| Lab |
Genetics
|
| Street address |
Schänzlestr. 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL8567 |
Agilent-021974 Syn_PCC6803_transcriptome |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA117187 |
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