|
| Status |
Public on Nov 01, 2022 |
| Title |
Gene expression in activated WT or PDK4 -/- CD4+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Activation of CD4+ T cells requires metabolic reprograming to sustain demands of cellular building blocks and ATP. Pyruvate dehydrogenase kinase (PDK) is an enzyme regulating pyruvate dehydrogenase complex subunit alpha 1 (PDHE1-alpha), which converts pyruvate to acetyl-CoA. Gene expression analysis of TCR-stimulated CD4+ T cells with PDK4 deletion exhibited the reduced calcium signaling and aerobic glycolysis pathway.
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| |
|
| Overall design |
Comparing gene expression of WT and PDK4 deficient CD4+ T cells
|
| |
|
| Contributor(s) |
Lee H, Kim ES, Lee I |
| Citation(s) |
36229019 |
| |
| Submission date |
Nov 17, 2020 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Hoyul Lee |
| E-mail(s) |
hoyul.lee@outlook.kr
|
| Organization name |
Kyungpook Nationatl University Hospital
|
| Department |
Bio-medical Research Institute
|
| Street address |
807, Hoguk-ro, Buk-gu
|
| City |
Daegu |
| ZIP/Postal code |
41404 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA679025 |
| SRA |
SRP292939 |
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