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| Status |
Public on Nov 26, 2020 |
| Title |
Modulation of the complex regulatory network for methionine biosynthesis in fungi |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, C. albicans regulates its methionine biosynthetic pathways using the transcription factors Met4 and Cbf1. This circuitry appears more complex than that of filamentous fungi, which use orthologs of the Met4 transcription factor, but lack versions of Cbf1, but is considerably less complex than that of S. cerevisiae, which 2 requires two transcription complexes, involving Cbf1 and Met31/32 as DNA binding modules, and Met4 and the Met4 ortholog Met28 as co-activators, to regulate Met biosynthesis. Although C. albicans contains Met31/32 and Met28 orthologs, they do not appear to be linked to regulation of the methionine circuit. Thus, the phylogenetic progression from filamentous fungi to S. cerevisiae is characterized by increasing complexity of the regulators, although the structural elements of the pathways are fundamentally the same.
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| Overall design |
Liquid cultures of all strains were adjusted to OD600 at 0.8 in 10 mL liquid YPD medium and incubated at 30 degrees with shaking at 220 rpm. We conduted RNAseq profiling for 2 replicates.
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| Contributor(s) |
Shrivastava M, Feng J, Coles M, Clark B, Islam A, Dumeaux V, Whiteway M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 25, 2020 |
| Last update date |
Nov 28, 2020 |
| Contact name |
Vanessa Dumeaux |
| E-mail(s) |
vanessadumeaux@gmail.com
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| Organization name |
Western University
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| Department |
Anatomy and Cell Biology
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| Street address |
Elgin Dr
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| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6G 2V4 |
| Country |
Canada |
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| Platforms (1) |
| GPL23041 |
Illumina MiSeq (Candida albicans) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA680785 |
| SRA |
SRP294169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162171_RAW.tar |
1.7 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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