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| Status |
Public on Dec 21, 2022 |
| Title |
Global transcriptome analysis of long RNA molecules expressed in human keratinocyte cell line HaCaT pre-treated with TGF-β1 in response to silymarin, a natural plant extract involved in skin tissue regeneration |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study is to find out the exact mechanisms of action of silymarin (SM), a complex mixture of flavonolignans structures isolated from milk thistle (Silybum marianum), on the biological behavior (phenotype) of human HaCaT keratinocytes.
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| Overall design |
To place the HaCaT cells in a context of a tissue re-epithelization process, we treated these cells for 24 hours with the Transforming Growth Factor β1 (TGF-β1, 10 ng/ml) in presence or absence of silymarin (SM, 31.25 µg/ml). Total RNA (longer than 200 nucleotides) were isolated, polyA RNA were enriched and sequenced from 3 biological replicate samples of TGF-β1-treated HaCaT cells (TGF) and 3 biological replicate samples of TGF-β1 plus SM-treated HaCaT cells (TGF.SM31).
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| Contributor(s) |
Henriet E, Baril P |
| Citation(s) |
36968096 |
| |
| Submission date |
Nov 30, 2020 |
| Last update date |
Apr 25, 2023 |
| Contact name |
Patrick Baril |
| E-mail(s) |
patrick.baril@cnrs.fr
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| Organization name |
Centre de Biophysique Moléculaire, CNRS-UPR4301
|
| Street address |
Rue Charles Sadron
|
| City |
Orléans |
| ZIP/Postal code |
45071 |
| Country |
France |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA681551 |
| SRA |
SRP295109 |
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