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Series GSE162341 Query DataSets for GSE162341
Status Public on Sep 21, 2023
Title Systematic phenotypic characterization and integrated analysis show heterogeneous neomorphic activities of different missense mutant p53 proteins
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The phenotypic and transcriptomic data on ten normal breast epithelial cell lines each expressing a distinct missense mutant p53 protein, and their systems-level analysis through ChIP-Seq and RNA-seq defines the molecular basis for phenotypic heterogeneity imparted by the different missense mutant p53 proteins. The focus of the study is to gain mechanistic insight on the neomorphic function of mutant p53 proteins and their manifestation into phenotypic heterogeneity in the context of a TNBC model.
Overall design Examining the transcriptomics of 10 different p53 missense mutant protein expressing MCF10A cell lines using RNA-seq, and DNA binding and promoter occupancy of 5 p53 mutant MCF10A cell lines using ChIP-Seq. The missense mutant p53 proteins have a V5 tag at the C-term and for ChIP, the mutant p53 proteins were pulled down using the V5 antibody. For the ChIP-seq, the MCF10A cells with endogenous wild type p53 served as the negative control and the cells expressing WT p53 with the V5 tag at C-term as the positive control.
Contributor(s) Pal A, Gonzalez-Malerva L, Xu C, Eaton S, Christensen G, Blain J, Mellor N, Steel J, Gupta C, Streitwieser E, Singharoy A, Park JG, LaBaer J
Citation(s) 37773066
Submission date Nov 30, 2020
Last update date Oct 19, 2023
Contact name Jin G Park
Organization name Arizona State University
Street address 727 E. Tyler St., Suite A220C
City Tempe
State/province AZ
ZIP/Postal code 85281-2115
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (23)
GSM4949987 MCF10A p53 Y234C mutant cells - RNA-seq
GSM4949988 MCF10A p53 H179R mutant cells - RNA-seq
GSM4949989 MCF10A p53 G245S mutant cells - RNA-seq
BioProject PRJNA681560
SRA SRP295117

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Supplementary file Size Download File type/resource
GSE162341_RAW.tar 820.0 Kb (http)(custom) TAR (of NARROWPEAK)
GSE162341_RNA_Seq_log2TPM.csv.gz 1.5 Mb (ftp)(http) CSV
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Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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