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| Status |
Public on Jul 13, 2021 |
| Title |
Genetic and Epigenetic Regulation of Skeletal Muscle Ribosome Biogenesis with Exercise |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Ribosomes are the macromolecular engines of protein synthesis. Factors mediating ribosome biogenesis in skeletal muscle that facilitate adaptation to exercise are multi-factorial, but may be related to ribosomal DNA (rDNA) dosage and/or epigenetic modifications to rDNA. To investigate these possibilities, we conducted reduced representation bisulfite sequencing (RRBS) on skeletal muscle biopsies from human subjects before and after exercise and to detect differences in methylation patterns during exercise adaptation.
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| Overall design |
Examination of DNA methylation in response to acute resistance exercise. Biopsies were obtaiend at rest, 30 min,and 24h after acute exercse. (n=10, 4 x 7 x 2 exercises); 3 control subjects received biopsies without exercise.
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| Contributor(s) |
Wen Y, Murach KA, von Walden F |
| Citation(s) |
33913170 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K99 AG063994 |
Myonuclear Epigenetics of Skeletal Muscle Mass Regulation with Age |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
Kevin Murach |
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| |
| Submission date |
Dec 01, 2020 |
| Last update date |
Jul 16, 2021 |
| Contact name |
Yuan Wen |
| E-mail(s) |
ywen2@g.uky.edu
|
| Phone |
8592186846
|
| Organization name |
University of Kentucky
|
| Street address |
760 Press Ave
|
| City |
Lexington |
| State/province |
Kentucky |
| ZIP/Postal code |
40536 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (33)
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| Relations |
| BioProject |
PRJNA681780 |
| SRA |
SRP295299 |
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