 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 30, 2021 |
| Title |
Role of Top1 and Genome structure in SARS-CoV-2 infection [RNA-Seq hamster] |
| Organism |
Mesocricetus auratus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The ongoing pandemic caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is currently affecting millions of lives worldwide. Large retrospective studies indicate that an elevated level of inflammatory cytokines and pro-inflammatory factors are associated with both increased disease severity and mortality. Targeting these pathways might therefore be a viable therapeutic strategy. Previously, we have reported that chromatin factors such as Topoisomerase I (Top1) play key roles in controlling the induction of inflammatory gene expression programs. Here, by using multidimensional epigenetic, transcriptional, in vitro and in vivo analyses, we show that Topoisomerase 1 (Top1) inhibition in infected cells and animals suppresses lethal inflammation induced by SARS-CoV-2.
|
| |
|
| Overall design |
To study the role of Top1 in modulating inflammation during SARS-CoV-2 in vivo, we infected golden syrian hamsters with SARS-CoV-2. Animals were dosed with either 10mg/kg TPT or vehicle control alone (DMSO) at Days 1 and 2 post infection. Thereafter, lungs of animals were collected at either day4 or day 6 post infection. All conditions were done with 3 animals (3 biological replicates).
|
| |
|
| Contributor(s) |
Ho JS, Mok BW, Campisi L, Jordan T, Yildiz S, Parameswaran S, Wayman JA, Gaudreault NN, Meekins DA, Indran V, Morozov , Trujillo D, Fstkchyan YS, Rathnasinghe R, Zhu , Zheng S, Zhao N, White K, Ray-Jones H, Malysheva V, Thiecke MJ, Lau S, Liu H, Zhang AJ, Lee AC, Liu , Aydillo , Melo S, Guccione E, Sebra , Shum , Bakker J, Kaufman DA, Moreira AL, Carossino , Balasuriya B, Byun , Miraldi ER, Albrecht RA, l Schotsaert , Garcia-Sastre A, Chanda SK, Jeyasekharan AD, TenOever R, Spivakov M, Weirauch MT, Heinz S, Chen H, Benner C, Richt JA, Marazzi I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 03, 2020 |
| Last update date |
Mar 30, 2021 |
| Contact name |
Sook Yuin Jessica Ho |
| E-mail(s) |
sook.ho@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Microbiology
|
| Lab |
Ivan Marazzi
|
| Street address |
1468 Madison Avenue
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10028 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24490 |
Illumina NextSeq 500 (Mesocricetus auratus) |
|
| Samples (15)
|
|
| This SubSeries is part of SuperSeries: |
| GSE162619 |
Role of Top1 and Genome structure in SARS-CoV-2 infection |
|
| Relations |
| BioProject |
PRJNA682410 |
| SRA |
SRP295688 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162618_exp-counts_hamster.txt.gz |
519.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...