|
| Status |
Public on Jan 12, 2021 |
| Title |
PDA Indolylmaleimides Induce Anti-Tumor Effects in Prostate Carcinoma Cell Lines through Mitotic Death |
| Organisms |
Homo sapiens; Canis lupus familiaris |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Introduction: Castrate resistant prostate cancer in men shares several characteristics with late-stage canine prostate cancer. Consequently, dogs became a model organism for the study of the human disease. Due to current insufficient therapies, evaluation of novel therapeutic agents for late-stage prostate cancer is of considerable interest for both species.
Methods: In this interdisciplinary study we characterized the influence of two indolylmaleimides (PDA-66 and PDA-377) on human and canine prostate carcinoma cell lines. For this purpose, we analyzed morphology, proliferation and apoptosis, recorded live cell imaging movies and performed whole transcriptome sequencing.
Results: While PDA-377 showed only weak growth inhibition on the human prostate cancer cell lines, PDA-66 inhibited proliferation and induced apoptosis in all prostate carcinoma cell lines with concentrations in the low micromolar range. Morphological characterization and whole transcriptome sequencing revealed that PDA-66 induces mitotic death through its microtubule-depolymerizing ability.
Discussion: PDA-66 appears to be a worthwhile anti-mitotic agent for further evaluation. The similarities in cellular and molecular response observed in the cell lines of both origins form a solid basis for the use of canine PCa in vivo models to provide valuable transferable findings to the benefit of both species.
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| Overall design |
Examination of the mediated effects by PDA-66 application on human and canine prostate carcinoma cell lines
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| |
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| Contributor(s) |
Schille JT, Nolte I, Beck J, Jilani D, Roolf C, Pews-Davtyan A, Rolfs A, Henze L, Beller M, Brenig B, Junghanss C, Schütz E, Murua Escobar H |
| Citation(s) |
33553272 |
| |
| Submission date |
Dec 08, 2020 |
| Last update date |
Apr 13, 2021 |
| Contact name |
Jan Torben Schille |
| Organization name |
University of Veterinary Medicine Hannover
|
| Department |
Small Animal Clinic
|
| Street address |
Buenteweg 9
|
| City |
Hannover |
| ZIP/Postal code |
30559 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL21400 |
Illumina NextSeq 500 (Canis lupus familiaris) |
|
| Samples (27)
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| Relations |
| BioProject |
PRJNA683222 |
| SRA |
SRP297007 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162832_CT1258_12h_vs_control.xlsx |
1.4 Mb |
(ftp)(http) |
XLSX |
| GSE162832_CT1258_24h_vs_control.xlsx |
1.4 Mb |
(ftp)(http) |
XLSX |
| GSE162832_CT1258_gene_counts.csv.gz |
230.9 Kb |
(ftp)(http) |
CSV |
| GSE162832_LNCaP_12h_vs_control.xlsx |
1.8 Mb |
(ftp)(http) |
XLSX |
| GSE162832_LNCaP_24h_vs_control.xlsx |
1.8 Mb |
(ftp)(http) |
XLSX |
| GSE162832_LNCaP_gene_counts.csv.gz |
320.5 Kb |
(ftp)(http) |
CSV |
| GSE162832_PC-3_12h_vs_control.xlsx |
1.6 Mb |
(ftp)(http) |
XLSX |
| GSE162832_PC-3_24h_vs_control.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
| GSE162832_PC-3_gene_counts_without_PD1.csv.gz |
313.4 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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