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Series GSE163003 Query DataSets for GSE163003
Status Public on Dec 11, 2020
Title Macrophage heterogenity in liver and adipose during obesity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Liver and adipose tissue undergo dynamic inflammatory changes during the progression of obesity. This study was designed to evaluate the changes in gene expression profile and function of subpecific macophage subsets.
Overall design Cx3Cr1-GFP mice were fed a STD or high fat, high sucrose diet for 16 weeks after which macrophages were isolated from the adipose tissue and liver. Flow sorting was utilized to divide liver macrophages into F4/80hi cells expressing TIM4 [i.e., STD KC, HFD KC] or those expressing Cx3cr1 (i.e., GFP positive) [Cx3cr1pos macs-HFD]. In the adipose tissue, high fat diet ATMs were flow sorted based on their expression of F4/80 and CD64 [i.e., ATMs-HFD].
A total of 3 samples from STD KC, 5 samples from HFD KCs, 4 samples of Cx3cr1 Macs, and 5 ATM samples on HFD were collected.
Contributor(s) Daemen S, Gainullina A, Kalugotla G, He L, Chan MM, Beals JW, Liss KH, Klein S, Feldstein AE, Artyomov MN, Finck BN, Schilling JD
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Submission date Dec 10, 2020
Last update date Dec 12, 2020
Contact name Maxim N. Artyomov
Organization name Washington University in St.Louis
Department Immunology&Pathology
Street address 660 S. Euclid Avenue, Campus Box 8118
City St.Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (17)
GSM4970031 std1_liv_frac_neg
GSM4970032 std2_liv_frac_neg
GSM4970033 std3_liv_frac_neg
BioProject PRJNA684317
SRA SRP297578

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Supplementary file Size Download File type/resource
GSE163003_all.gene_counts.xls.gz 2.9 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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