 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 18, 2021 |
| Title |
Genome-wide maps of chromatin state in PANC-1 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate functional associations between SIRT1 and CUL4B, we used ChIP-seq to analyze genome-wide SIRT1/CUL4B complex transcriptional targets. ChIP experiments were performed first in PANC-1 cells using antibodies against SIRT1 or CUL4B. Next, SIRT1- or CUL4B-associated DNA was amplified using non-biased conditions, labeled, and sequenced. Using Illumina HiSeq2000, we found lots of SIRT1- and CUL4B-specific binding peaks, respectively, representing the ChIP-seq peak. We found that strong enrichment on the promoters of selected genes involved in classical pathways. This study gave us a new understanding of the role of SIRT1/CUL4B in chromatin status and gene transcription.
|
| |
|
| Overall design |
Examination of SIRT1 and CUL4B target genes in PANC-1 cell line
|
| |
|
| Contributor(s) |
Leng S, Wang Y |
| Citation(s) |
34163012 |
| |
| Submission date |
Dec 16, 2020 |
| Last update date |
Jun 30, 2021 |
| Contact name |
yan wang |
| E-mail(s) |
yanwang@tmu.edu.cn
|
| Organization name |
National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences
|
| Street address |
17 panjiayuan nanli, chaoyang district, Beijing, China
|
| City |
beijing |
| ZIP/Postal code |
100021 |
| Country |
Zimbabwe |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA685768 |
| SRA |
SRP298158 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163337_RAW.tar |
1.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
