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Series GSE163440 Query DataSets for GSE163440
Status Public on Feb 22, 2021
Title IL-21 and IFNalpha therapy rescues terminally-differentiated NK cells and limit SIV reservoir in ART-treated macaques [cohort1]
Organism Macaca mulatta
Experiment type Expression profiling by high throughput sequencing
Summary Unlike HIV infection, which progresses to AIDS absent suppressive anti-retroviral therapy (ART), nonpathogenic infections in natural hosts, such African green monkeys (AGMs), are characterized by a lack of gut microbial translocation and robust secondary lymphoid Natural Killer (NK) cell responses resulting in absence of chronic inflammation and limited SIV dissemination in lymph nodes (LN) B-cell-follicles, respectively. In a pathogenic infection model (i.e. ART-treated, SIVmac239-infected rhesus macaques; RMs), sequential Interleukin (IL)-21 and interferon (IFN)-alpha therapy generated terminally-differentiated blood NK cells (NKG2a/clowCD16+) with potent HLA-E-restricted activity in response to SIV-ENV peptides in contrast to control RMs, where less differentiated, IFN-gamma+ NK cells predominated. The frequency and cytolytic activity of NKG2a/clowCD16+ NK cells correlated with a reduction of replication-competent SIV in LN during ART and viral rebound delay following analytical treatment interruption. These data demonstrate that AGM-like NK cell differentiation profiles can be rescued in RMs to promote viral clearance in tissues.
 
Overall design 11 SIV infected and ART-treated rhesus macaques were used. 6 were treated with IL-21 and IFN-alpha and 5 were untreated controls.
 
Contributor(s) Harper J, Huot N, Micci L, Tharp GK, King C, Rascle P, Shenvi N, Wang H, Galardi C, Upadhyay AA, Villinger F, Lifson J, Easley K, Jacquelin B, Dunham RM, Bosinger S, Müller-Trutwin M, Paiardini M
Citation(s) 34001890
Submission date Dec 17, 2020
Last update date Jun 09, 2021
Contact name Gregory K Tharp
E-mail(s) gktharp@emory.edu
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
 
Platforms (1)
GPL23804 Illumina HiSeq 3000 (Macaca mulatta)
Samples (11)
GSM4979332 1_RWm11_7-19-16
GSM4979333 2_219_03_7-19-16
GSM4979334 3_RWc11_7-19-16
This SubSeries is part of SuperSeries:
GSE163443 IL-21 and IFNalpha therapy rescues terminally-differentiated NK cells and limit SIV reservoir in ART-treated macaques
Relations
BioProject PRJNA686107
SRA SRP298355

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Supplementary file Size Download File type/resource
GSE163440_Mirko_7-19-16_Raw_Counts.txt.gz 320.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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