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| Status |
Public on Jan 01, 2010 |
| Title |
Transcriptional analysis of the Neisseria gonorrhoeae Fur regulon |
| Platform organisms |
Neisseria gonorrhoeae; Neisseria meningitidis |
| Sample organism |
Neisseria gonorrhoeae FA 1090 |
| Experiment type |
Expression profiling by array
|
| Summary |
In Neisseria gonorrhoeae, Fur (ferric uptake regulator) protein regulates iron homeostasis gene expression through binding to conserved sequences in promoters of iron-responsive genes. We have expanded the gonococcal Fur regulon using a custom microarray to monitor iron-responsive gene expression throughout the growth curve combined with a genome-wide in silico analysis to predict Fur boxes (FB), and in vivo FuRTA assays to detect genes able to bind Fur.
Keywords: time course: (1hr ,2hr, 3hr, 4hr)
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| Overall design |
The effect of iron on global gene expression was determined over a 4-h time period by comparing the RNA profile of the organism grown in iron-deplete conditions to growth in iron-replete conditions.
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| Contributor(s) |
Jackson L, Ducey T, Zaitshik J, Orvis J, Dyer D |
| Citation(s) |
19854902 |
| |
| Submission date |
May 31, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Lydgia Jackson |
| E-mail(s) |
lydgia-jackson@ouhsc.edu, david-dyer@ouhsc.edu
|
| Phone |
405 271-1201
|
| Fax |
405 271-1204
|
| Organization name |
University of Oklahoma Health Sciences Center
|
| Department |
Microbiology and Immunology
|
| Lab |
Dyer
|
| Street address |
975 NE 10th Street
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL7320 |
OUHSC Dyer Neisseria gonorrhoeae 2K MPAUT1a520274F |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA115309 |
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