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| Status |
Public on Oct 29, 2022 |
| Title |
RNA-seq of injured mouse hearts depleted for myofibroblast Yap and Wwtr1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A myofibroblast specific tamoxifen inducible Cre was used to assess the role of Yap and Wwtr1 in the mouse heart after myocardial infarction
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| Overall design |
We implemented a Cre-lox system whereby inducible Cre expressed under the myofibroblast specific Periostin promoter is crossed with Yap and Wwtr1 floxed animals, facilitating inducible depletion of Yap (both alleles) and Wwtr1 (one allele). Mice were siblings with floxed Yap/Wwtr1 alleles and either a copy of MerCreMer under the endogenous periostin promote or wildtype Periostin. Permenant ligation of the left anterior descending artery was used to produce a surgical myocardial infarction. Mice were administered tamoxifen citrate diet ad libitum immediately after surgery to promote excession of myofibroblast yap and left ventricles were collected 4 days post injury.
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| Contributor(s) |
Flinn MA, Patterson M, CO'Meara C |
| Citation(s) |
36998974 |
| |
| Submission date |
Dec 21, 2020 |
| Last update date |
Apr 06, 2023 |
| Contact name |
Michael Andrew Flinn |
| Organization name |
Medical College of Wisconsin
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| Department |
Physiology
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| Lab |
O'Meara Lab
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| Street address |
8701 W Watertown Plank Rd
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| City |
Wauwatosa |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE163617 |
RNA-seq of injured mouse hearts depleted for myofibroblast Yap or Yap and Wwtr1 |
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| Relations |
| BioProject |
PRJNA686945 |
| SRA |
SRP298705 |
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