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| Status |
Public on Jun 02, 2009 |
| Title |
Microarray Analysis of Lymphatic Tissue Reveals Stage-Specific, Gene-Expression Signatures in HIV-1 Infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Untreated HIV-1 infection progresses through acute and asymptomatic stages to AIDS. While each of the three stages has well-known clinical, virologic and immunological characteristics, much less is known of the molecular mechanisms underlying each stage. Here we report lymphatic tissue microarray analyses revealing for the first time stage-specific patterns of gene expression during HIV-1 infection. We show that while there is a common set of key genes with altered expression throughout all stages, each stage has a unique gene-expression signature. The acute stage is most notably characterized by increased expression of hundreds of genes involved in immune activation, innate immune defenses (e.g.MDA-5, TLR-7 and -8, PKR, APOBEC3B, 3F, 3G), adaptive immunity, and in the pro-apoptotic Fas-Fas-L pathway. Yet, quite strikingly, the expression of nearly all acute-stage genes return to baseline levels in the asymptomatic stage, accompanying partial control of infection. In the AIDS stage, decreased expression of numerous genes involved in T cell signaling identifies genes contributing to T cell dysfunction. These common and stage-specific, gene-expression signatures provide new insights into the molecular mechanisms underlying the host response and the slow, natural course of HIV-1 infection.
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| Overall design |
A total of 52 patients were analyzed: 10 patients unaffected, 18 patients with asymptomatic stages of AIDs, 16 patients with acute stages of AIDs, and 8 patients with AIDs.
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| Contributor(s) |
Li Q, Smith AJ, Schacker TW, Carlis JV, Duan L, Reilly CS, Haase AT |
| Citation(s) |
19596987, 21525393 |
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| Submission date |
Jun 01, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
qingsheng li |
| E-mail(s) |
lixxx076@umn.edu
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| Phone |
612-624-9130
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| Fax |
612-626-0623
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| Organization name |
University of Minnesota
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| Department |
Microbiology
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| Lab |
Ashley T. Haase
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| Street address |
Box 196, 420 Delaware ST SE
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| City |
minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (52)
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| Relations |
| BioProject |
PRJNA115319 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16363_RAW.tar |
448.3 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE16363_further_processed data.xls.gz |
4.0 Mb |
(ftp)(http) |
XLS |
| Processed data included within Sample table |
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