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| Status |
Public on Jul 08, 2022 |
| Title |
key histone modifications in porcine oocytes and early embryos |
| Organism |
Sus scrofa |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Conservation and species specificity are both unveiled in the reprogramming process of epigenetic modifications in early embryos. Pig is important agricultural livestock that closely related to human life and understanding the mechanisms of epigenetic reprogramming in porcine embryos could be assistive to the pig breeding industry and also informative to human embryo research. Nevertheless, current knowledge of epigenetic reprogramming in porcine embryos is elusive. We profile H3K4me3, H3K27me3, and H3K27ac modifications in porcine oocytes and preimplant embryos. Our data reveal the existence of unusual broad H3K4me3 domains not only in oocytes but also expanded in pre-ZGA embryos and demonstrates the extensive transitions of unusual broad H3K4me3 domains and their roles during embryogenesis. Notably, broad H3K27me3 and H3K27ac enriched domains are found to be preferably colocalized in these broad H3K4me3 domains when comparing to it in mice and human. By knocking down KDM5B in pig embryos, cleavage and developments were disrupted as demonstrated in mouse.
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| Overall design |
Oocytes (GV and MII stages) and IVF embryos (zygote, 2-cell, 4-cell, 8cell, morula and blastocyst) were collected and H3K4me3 (Abcam, ab8580) and H3K27me3 (Millipore, 07449) were profiled with ULI-NChIP protocol. In addition, to investigate the functions of KDM5B/C during pig embryogenesis, siRNA targeting pig KDM5B/C were injected into zygotes and then embryos at 4-cell stages were collected for H3K4me3 ChIP-seq experiments. For each developing stage or condition, two replicates were performed for each histone modification.
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| Contributor(s) |
Bu G, Zhu W, Liu X, Miao Y |
| Citation(s) |
35868641 |
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| Submission date |
Dec 22, 2020 |
| Last update date |
Oct 12, 2022 |
| Contact name |
Wei Zhu |
| E-mail(s) |
zhuwei@mail.hzau.edu.cn
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| Organization name |
Huazhong Agricultural University
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| Lab |
Yi-Liang Miao Lab
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| Street address |
No.1,Shizishan Street , Hongshan District
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| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA687245 |
| SRA |
SRP298945 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163709_RAW.tar |
3.4 Gb |
(http)(custom) |
TAR (of BROADPEAK, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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