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| Status |
Public on Jan 01, 2011 |
| Title |
Gene expression profiling in Salmonella Typhimurium encountered with heterophils |
| Platform organism |
Salmonella enterica |
| Sample organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Expression profiling by array
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| Summary |
To study the pathogenicity of Salmonella Typhimurium (ST) in heterophils, a whole Salmonella genome array was used to analyze RNA isolated from Salmonella in vitro encountered with heterophils (30 min, 1 h, 2 h, and 3 h) or medium control (NS). Dual-color, direct comparisons were carried out between heterophils-encountered ST and non-encountered controls (30 min vs. NS (30M/NS), 1 h vs. NS (1HR/NS), 2 h vs. NS (2HR/NS) and 3 h vs. NS (3HR/NS)). Each comparison includes four biological replicates. Out of 4588 genes on an array, there were 605, 758, 944 and 945 genes differentially expressed in the comparisons of 30M/NS, 1HR/NS, 2HR/NS and 3HR/NS, respectively (P < 0.001, fold-change > 2). Most of the Salmonella genes associated with invasion function were found differentially expressed at 2HR/NS and 3HR/NS comparisons. These candidate genes include TypeIII-secreted protein effector sopE2, Invasion proteins (e.g. invA, invE, invG, invH), and Invasion genes tranction activator hilA. The results of continuous transcriptional changes provide important information to elucidate the pathogenicity of ST in heterophils.
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| Overall design |
Dual-color, common reference comparisons were carried out between heterophils-encountered ST and non-encountered controls (30 min vs. NS (30M/NS), 1 h vs. NS (1HR/NS), 2 h vs. NS (2HR/NS) and 3 h vs. NS (3HR/NS)). Four biological replicates, with dye swap labeling, were included in each of comparisons. Background subtracted signal intensity were collected from 16 arrays and normalized before data analysis.
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| Contributor(s) |
Chiang H, Swaggerty CL, Kogut MH, Dowd SE, Zhou H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 03, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Huaijun Zhou |
| Organization name |
Texas A&M University
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| Street address |
Rm 418D Kleberg Center,Texas A&M University
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| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843-2472 |
| Country |
USA |
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| Platforms (1) |
| GPL8140 |
Salmonella whole-genome custom Agilent array |
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| Samples (16)
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| GSM410630 |
ST-Host Project_ST array_S1A1_(030vs000) |
| GSM410897 |
ST-Host Project_ST array_S1A2_(000vs030) |
| GSM410898 |
ST-Host Project_ST array_S1A3_(030vs000) |
| GSM410899 |
ST-Host Project_ST array_S1A4_(000vs030) |
| GSM410900 |
ST-Host Project_ST array_S1A5_(060vs000) |
| GSM410901 |
ST-Host Project_ST array_S1A6_(000vs060) |
| GSM410902 |
ST-Host Project_ST array_S1A7_(060vs000) |
| GSM410903 |
ST-Host Project_ST array_S1A8_(000vs060) |
| GSM410904 |
ST-Host Project_ST array_S2A1_(120vs000) |
| GSM410905 |
ST-Host Project_ST array_S2A2_(000vs120) |
| GSM410906 |
ST-Host Project_ST array_S2A3_(120vs000) |
| GSM410907 |
ST-Host Project_ST array_S2A4_(000vs120) |
| GSM410908 |
ST-Host Project_ST array_S2A5_(180vs000) |
| GSM410909 |
ST-Host Project_ST array_S2A6_(000vs180) |
| GSM410910 |
ST-Host Project_ST array_S2A7_(180vs000) |
| GSM410911 |
ST-Host Project_ST array_S2A8_(000vs180) |
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| Relations |
| BioProject |
PRJNA116289 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16399_RAW.tar |
23.3 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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