 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2024 |
| Title |
PDGFRα+ not CD137+ progenitors control visceral obesity via glucocorticoid mediated TEAD1 methylation state |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
summary:Glucocorticoids have impact on visceral obese which crucial contributor to the burden of obesity complications. Subcutaneous and visceral adipose have the heterogeneity of cellular populations. However, the mechanism of glucocorticoids mediated depot-specific regulation remains unclear. Here, we report PDGFRα+ progenitors, dominated in visceral adipose which can convert to white-typical adipocytes, are glucocorticoid-sensitive progenitors. While CD137+ progenitors, mainly harbored in subcutaneous adipose, which are beige-typical adipocytes, are glucocorticoid-passivated progenitors. Mechanistically, we proved that Glucocorticoids elevate TEAD1, transcriptional coactivator of glucocorticoid receptor, by down-regulating the methylation states in PDGFRα+ progenitors. TEAD1 bonds the accessible binding sites in miR-27b promoter region and transcriptionally increases miR-27b expression which previously proved to be the browning ability inhibitor in human and rodent white adipose. These data provide insight into the mechanism of glucocorticoids mediation of visceral obesity and the pathophysiology of depot-specific variations of high fat diet induced obesity.
|
| |
|
| Overall design |
two samles
|
| |
|
| Contributor(s) |
Lv Y, Ding G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 03, 2021 |
| Last update date |
Jan 01, 2024 |
| Contact name |
yifan lv |
| Organization name |
the First Affiliated Hospital to Nanjing Medical University
|
| Department |
Department of Geriatrics
|
| Street address |
300 Guangzhou Road
|
| City |
nanjing |
| State/province |
jiangsu |
| ZIP/Postal code |
210029 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA689301 |
| SRA |
SRP300086 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE164154_CD140VsCD137_DMR.bed.gz |
563.8 Kb |
(ftp)(http) |
BED |
| GSE164154_RAW.tar |
550.9 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
