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| Status |
Public on Sep 09, 2011 |
| Title |
Expression data from human primary fibroblasts treated with Trypanosoma cruzi-conditioned medium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The intracellular pathogen Trypanosoma cruzi secretes an activity that blocks TGF-β-dependent induction of connective tissue growth factor (CTGF/CCN2). Here, we address the mechanistic basis for T. cruzi-mediated interference of
CTGF/CCN2 expression by examining host cell signaling pathways and the global inhibitory effect on TGF-β-dependent gene expression. We show that the expression of a discrete subset of TGF-β-inducible genes involved in cell proliferation, wound repair, and immune regulation are blocked by the soluble T. cruzi activity, demonstrating that this parasite-derived activity has broad, but specific effects on fibroblast gene regulation.
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| Overall design |
Primary human fibroblasts were treated with TGF-β, T. cruzi conditioned medium (PCM) and TGF-β/ PCM simultaneously. Untreated cells were also included as controls. Total RNA was extracted and gene expression levels analyzed with Affymetrix microarrays. Three independent biological replicates were included for each type of treatment.
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| |
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| Contributor(s) |
Mott GA, Costales JA, Burleigh BA |
| Citation(s) |
21931601 |
| |
| Submission date |
Jun 03, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jaime Alfredo Costales |
| E-mail(s) |
jacostalesc@puce.edu.ec, jaimecostales@gmail.com
|
| Organization name |
Pontificia Universidad Catolica del Ecuador
|
| Department |
Biological Sciences
|
| Lab |
Center for Infectious Disease Research
|
| Street address |
12 de Octubre y Patria
|
| City |
Quito |
| ZIP/Postal code |
NN |
| Country |
Ecuador |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (12)
|
| GSM412619 |
Cells treated with medium as control, biological rep 1 |
| GSM412620 |
Cells treated with medium as control, biological rep 2 |
| GSM412621 |
Cells treated with medium as control, biological rep 3 |
| GSM412622 |
Cells treated with parasite conditioned medium, biological rep 1 |
| GSM412623 |
Cells treated with parasite conditioned medium, biological rep 2 |
| GSM412624 |
Cells treated with parasite conditioned medium, biological rep 3 |
| GSM412625 |
Cells treated with TGF, biological rep 1 |
| GSM412626 |
Cells treated with TGF, biological rep 2 |
| GSM412627 |
Cells treated with TGF, biological rep 3 |
| GSM412628 |
Cells treated with TGF and parasite conditioned medium, biological rep 1 |
| GSM412629 |
Cells treated with TGF and parasite conditioned medium, biological rep 2 |
| GSM412630 |
Cells treated with TGF and parasite conditioned medium, biological rep 3 |
|
| Relations |
| BioProject |
PRJNA116357 |
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