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| Status |
Public on Feb 22, 2021 |
| Title |
Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interleukin (IL)-11 is a member of the IL-6 family of cytokines and involved in multiple cellular responses, including tumor development. However, the origin and functions of IL-11-producing (IL-11+) cells are not fully understood. To characterize IL-11+ cells in vivo, we generated Il11 reporter mice. IL-11+ cells appeared in the colon of murine tumor and acute colitis models. Il11ra1 or Il11 deletion attenuated the development of colitis-associated colorectal cancer. IL-11+ cells expressed fibroblast markers, and genes associated with cell proliferation and tissue repair. IL-11 induced the activation of colonic fibroblasts and epithelial cells through phosphorylation of STAT3. Human cancer database analysis revealed that a set of genes enriched in IL-11+ fibroblasts was elevated in human colorectal cancer, and correlated with reduced recurrence-free survival. Together, IL-11+ fibroblasts activate both tumor cells and fibroblasts via secretion of IL-11, thereby constituting a feed forward loop between tumor cells and fibroblasts in the tumor microenvironment.
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| Overall design |
To characterize IL-11+ fibroblasts that appeared in colitis, we used a cell sorter to isolate EGFP+ and EGFP- fibroblasts from the colon of DSS-treated Il11-Egfp reporter mice and compared their gene expression profiles by RNA-seq analysis.
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| Contributor(s) |
Nishina T, Nakano H |
| Citation(s) |
33863879 |
| |
| Submission date |
Feb 19, 2021 |
| Last update date |
May 13, 2021 |
| Contact name |
Takashi Nishina |
| E-mail(s) |
takashi.nishina@med.toho-u.ac.jp
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| Phone |
+81-3-3762-4151
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| Organization name |
Toho University
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| Department |
Biochemistry
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| Street address |
5-21-16 Omori-nishi
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| City |
Ota-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
143-8540 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE141644 |
Characterization of Interleukin 11-producing fibroblasts that constitute a niche for tumor development |
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| Relations |
| BioProject |
PRJNA703020 |
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