 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2021 |
| Title |
RNA sequencing of Chlamydia trachomatis-stimulated HIV-1-infected CD4 T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sexually transmitted infections (STIs) are commonly reported among HIV-1 infected patients. The increasing prevalence of the most common STI, Chlamydia trachomatis (CT), among HIV-1 infected people suggests a role in HIV-1 infectivity. However, the mechanisms modulating the enhancement of HIV-1 infectivity during HIV-1/STIs coinfection remain elusive. The stimulation of CD4 T cells during CT infection may modulate the expression of specific genes, which in turn enhance the susceptibility and infectivity of CT-specific CD4 T cells to HIV-1 infection. After three days of CT stimulation of PBMCs followed by 3 days of HIV-1 infection, we observed a significant increase in HIV-1 p24 levels among clinically diagnosed C. trachomatis-infected patients as compared to cells from healthy donors. Similarly, ex vivo CT antigen-stimulated PBMCs from healthy donors showed enhanced susceptibility to HIV-1 as compared to unstimulated PBMCs. CT-specific CD4 T cells also harbour more HIV-1 copy numbers as compared to healthy unstimulated CD4 T cells. RNA-seq data revealed the upregulation of CCR chemokine receptors and cytokines in CD4 T cells from CT-stimulated CD4 T cells infected with HIV-1.
|
| |
|
| Overall design |
PBMCs from healthy blood donors were extracted and stimulated with Chlamydia trachomatis (CT) for three days followed by infection with 50ng/ml of HIV-1 p24 for three days. CD4+ T cells were isolated using the EasySep Human CD4+ T cells isolation kit (Stemcell Technologies, Canada) according to the manufacturer’s instruction followed by RNA extraction. Total RNAs from CT-HIV-1, HIV-1 only, and negative controls were used for RNA-seq at the Beijing Genome Institute (China).
|
| |
|
| Contributor(s) |
Dzakah EE, Zhao J, Wang L, Huang L, Wan Z, Rashid F, Wang H, Xue R, Chen S, Yang B, Deng K, Tang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 05, 2021 |
| Last update date |
Dec 31, 2021 |
| Contact name |
Emmanuel E Dzakah |
| E-mail(s) |
edzakah@mail.ustc.edu.cn
|
| Phone |
+8615665697192
|
| Organization name |
Dermatology Hospital
|
| Street address |
Lujing Lu, Yuexiu District
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510091 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA689763 |
| SRA |
SRP300411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE164264_CT_HIV_CD4.xls.xlsx |
1.3 Mb |
(ftp)(http) |
XLSX |
| GSE164264_HIV_CD4.xls.xlsx |
1.3 Mb |
(ftp)(http) |
XLSX |
| GSE164264_NC_CD4.xls.gz |
732.8 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...