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Status |
Public on Mar 21, 2022 |
Title |
Disordered N-Terminal DNMT3A Domain Is Required for Mouse Postnatal Development |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Methylation profiling by high throughput sequencing
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Summary |
DNA methyltransferase 3a (DNMT3A) plays a crucial role in multiple biological processes during mouse development. We report that the longer isoform DNMT3A1, but not the shorter DNMT3A2, is essential for mouse postnatal development. DNMT3A1 binds to and regulates bivalent developmental genes in the brain. The disordered N-terminal domain is required for DNMT3A1-regulated development, DNA methylation and gene expression in the nervous system. A potential ubiquitin-interacting motif (UIM) in the N-terminus may bind to histone H2AK119 ubiquitination, mediating the recruitment of DNMT3A1 to its targets. These data reveal the N-terminus as a necessary regulatory domain for DNMT3A1 chromatin occupancy and functions.
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Overall design |
Comparation of transcriptomes and DNA methylomes between WT and Dnmt3a mutant mouse cortex / neuron nuclei; Examination of chromatin binding profiles of DNMT3A isoforms and mutants
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Contributor(s) |
Gu T, Hao D, Goodell MA |
Citation(s) |
35534561 |
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Submission date |
Jan 05, 2021 |
Last update date |
Jun 20, 2022 |
Contact name |
Tianpeng Gu |
E-mail(s) |
tianpeng.gu@bcm.edu
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Organization name |
Baylor College of Medicine
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Street address |
Cambridge steet
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City |
Houston |
ZIP/Postal code |
77054 |
Country |
USA |
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Platforms (3) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
GPL30172 |
NextSeq 2000 (Mus musculus) |
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Samples (75)
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Relations |
BioProject |
PRJNA689766 |
SRA |
SRP300442 |