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Series GSE164265 Query DataSets for GSE164265
Status Public on Mar 21, 2022
Title Disordered N-Terminal DNMT3A Domain Is Required for Mouse Postnatal Development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary DNA methyltransferase 3a (DNMT3A) plays a crucial role in multiple biological processes during mouse development. We report that the longer isoform DNMT3A1, but not the shorter DNMT3A2, is essential for mouse postnatal development. DNMT3A1 binds to and regulates bivalent developmental genes in the brain. The disordered N-terminal domain is required for DNMT3A1-regulated development, DNA methylation and gene expression in the nervous system. A potential ubiquitin-interacting motif (UIM) in the N-terminus may bind to histone H2AK119 ubiquitination, mediating the recruitment of DNMT3A1 to its targets. These data reveal the N-terminus as a necessary regulatory domain for DNMT3A1 chromatin occupancy and functions.
 
Overall design Comparation of transcriptomes and DNA methylomes between WT and Dnmt3a mutant mouse cortex / neuron nuclei; Examination of chromatin binding profiles of DNMT3A isoforms and mutants
 
Contributor(s) Gu T, Hao D, Goodell MA
Citation(s) 35534561
Submission date Jan 05, 2021
Last update date Jun 20, 2022
Contact name Tianpeng Gu
E-mail(s) tianpeng.gu@bcm.edu
Organization name Baylor College of Medicine
Street address Cambridge steet
City Houston
ZIP/Postal code 77054
Country USA
 
Platforms (3)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL30172 NextSeq 2000 (Mus musculus)
Samples (75)
GSM5005395 WT_Cortex_input
GSM5005396 WT_Cortex_DNMT3A-FLAG
GSM5005397 WT_Cortex_H3K4me3
Relations
BioProject PRJNA689766
SRA SRP300442

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE164265_RAW.tar 8.2 Gb (http)(custom) TAR (of BEDGRAPH, BW, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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