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| Status |
Public on Jun 30, 2021 |
| Title |
RNA sequencing-based analysis of transcriptional reprogramming of primary human monocyte-derived macrophages infected with Mycobacterium tuberculosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Primary human monocytes were isolated from four healthy human blood donors. Monocytes were isolated from PBMC buffy coats using plastic adherence for 4 hours. Monocytes were allowed to differentiate into macrophages over a period of 1 week. Macrophages from each of the 4 donors were split into two groups - uninfected and infected with Mycobacterium tuberculosis (Mtb). Cells in the infection group were infected with Mtb for 48 hours at a multiplicity of infection (MOI) of 10. Following incubation, uninfected and infected cells were harvested for RNA. RNA was used for next-generation RNA sequencing. Raw RNA sequencing data was processed using a HISAT2, Stringtie, Ballgown, DESeq2 pipeline. Processed data was used to measure differential expression between uninfected and infected macrophages.
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| |
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| Overall design |
RNA sequencing of primary human monocyte-derived macrophages infected with Mtb, strain H37Rv for 48 hours at a MOI of 10.
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| Contributor(s) |
Looney MM, Karakousis PC |
| Citation(s) |
34140955 |
| |
| Submission date |
Jan 05, 2021 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Monika Looney |
| E-mail(s) |
mlooney2@jhmi.edu
|
| Organization name |
Johns Hopkins University
|
| Street address |
1503 E Jefferson Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA689801 |
| SRA |
SRP300490 |
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