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Status |
Public on Jan 09, 2021 |
Title |
Transcriptomic Response of the Diazotrophic Bacteria Gluconacetobacter diazotrophicus Strain PAL5 to Iron Limitation and Characterization of the fur Regulatory Network |
Organism |
Gluconacetobacter diazotrophicus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Gluconacetobacter diazotrophicus has been the focus of several studies aiming to understand the mechanisms behind this endophytic diazotrophic bacterium. The present study is the first global analysis of the early transcriptional response of exponentially growing G. diazotrophicus to iron, an essential cofactor for many enzymes involved in various metabolic pathways. RNA-seq, targeted gene mutagenesis and computational motif discovery tools were used to define the G. diazotrophicus Fur regulon. The data analysis showed that genes encoding functions related to iron homeostasis, were significantly upregulated in response to iron limitation. Certain genes involved in the secondary metabolism were overexpressed under iron-limited conditions. In contrast, it was observed that the expression of genes involved in Fe-S cluster biosynthesis, flagellar biosynthesis and type IV secretion systems were downregulated in an iron-depleted culture medium. Our results support a model that control transcription in G. diazotrophicus by Fur function. The G. diazotrophicus Fur protein was able to complement an E. coli fur mutant. These results provide new insights into the effects of iron on the metabolism of G. diazotrophicus, as well as demonstrating the essentiality of this micronutrient for the main characteristic of plant growth promotion by G. diazotrophicus.
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Overall design |
Profile mRNA obtained after half an hour incubation in the presence and absence of iron in triplicate.
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Contributor(s) |
de Paula Soares C, Trada-Sfeir MZ, Terra LA, de Paula Ferreira J, Araújo JL, Meneses CH, de Souza EM, Baldani JI, Schwab S |
Citation(s) |
35955667 |
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Submission date |
Jan 08, 2021 |
Last update date |
Aug 24, 2022 |
Contact name |
Leonardo Araujo Terra |
E-mail(s) |
leonardoterra@hotmail.com.br
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Organization name |
Embrapa Agrobiologia
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Lab |
Genetics and biochemistry laboratory
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Street address |
Rodovia BR-465
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City |
Seropédica |
State/province |
RJ |
ZIP/Postal code |
23897-970 |
Country |
Brazil |
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Platforms (1) |
GPL29580 |
Ion Torrent PGM (Gluconacetobacter diazotrophicus) |
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Samples (6)
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GSM5009685 |
G. diazotrophicus cells, 37 µM FeCl3 R1 |
GSM5009686 |
G. diazotrophicus cells, 37 µM FeCl3 R2 |
GSM5009687 |
G. diazotrophicus cells, 37 µM FeCl3 R3 |
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Relations |
BioProject |
PRJNA690722 |
SRA |
SRP300890 |