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| Status |
Public on Aug 06, 2013 |
| Title |
NOTCH3 is a master-regulator of motility in neuroblastoma and is essential for cell survival |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Migratory embryonal neuroblasts give rise to several tissues, including the sympathetic nervous system (SNS). Neuroblastomas are paediatric tumours of the peripheral SNS with a highly variable prognosis. We observed that high NOTCH3 expression in neuroblastomas correlated with a poor prognosis. Expression of a NOTCH3 transgene in neuroblastoma cells induced many motility genes and conferred a highly motile phenotype. Expression of these motility genes strongly correlated with NOTCH3 expression in neuroblastomas and many other tumours, suggesting a general role for NOTCH3 in regulation of these genes. Silencing of NOTCH3 or genes of the Notch-processing γ-secretase complex induced apoptosis in all neuroblastoma cell lines tested. These data suggest that NOTCH3 is a key-regulator of motility, and indispensable for survival of neuroblastoma cells.
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| Overall design |
Time-course experiments of NOTCH3IC induction in two independent clones (c6 and c8), with 9 or 8 time points, respectively.
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| Contributor(s) |
van Nes J, Koster J, Versteeg R |
| Citation(s) |
23649002, 25315710 |
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| Submission date |
Jun 07, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Richard Volckmann |
| E-mail(s) |
r.volckmann@amsterdamumc.nl
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| Organization name |
Academic Medical Centre, University of Amsterdam
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| Department |
Department of Oncogenomics
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| Street address |
P.O. Box 22700
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| City |
Amsterdam |
| ZIP/Postal code |
1100DE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (17)
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| GSM414094 |
IMR32, c6, NOTCH3IC, t=12 |
| GSM414095 |
IMR32, c6, NOTCH3IC, t=24 |
| GSM414096 |
IMR32, c6, NOTCH3IC, t=48 |
| GSM414097 |
IMR32, c6, NOTCH3IC, t=72 |
| GSM414098 |
IMR32, c6, NOTCH3IC, t=96 |
| GSM414099 |
IMR32, c6, NOTCH3IC, t=120 |
| GSM414100 |
IMR32, c8, NOTCH3IC, t=0 |
| GSM414101 |
IMR32, c8, NOTCH3IC, t=4 |
| GSM414102 |
IMR32, c8, NOTCH3IC, t=8 |
| GSM414103 |
IMR32, c8, NOTCH3IC, t=12 |
| GSM414104 |
IMR32, c8, NOTCH3IC, t=24 |
| GSM414105 |
IMR32, c8, NOTCH3IC, t=48 |
| GSM414106 |
IMR32, c8, NOTCH3IC, t=72 |
| GSM414107 |
IMR32, c8, NOTCH3IC, t=96 |
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| This SubSeries is part of SuperSeries: |
| GSE16254 |
Integrated bioinformatic and wet-lab approach to identify potential oncogenic networks in neuroblastoma and other tumors |
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| Relations |
| BioProject |
PRJNA122891 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16477_RAW.tar |
77.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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