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Series GSE16488

Query DataSets for GSE16488

Status

Public on Jan 25, 2010

Title

growth dependent expression of s. aureus mssa476

Organism

Staphylococcus aureus subsp. aureus MSSA476

Experiment type

Expression profiling by array

Summary

Survival and pathogenesis of Staphylococcus aureus in the host requires the ability to respond to changes. Therefore, tight regulation of gene expression via various regulators is essential. Also, the organization of genes in operons is of influence on the regulation of gene expression. Knowledge of gene expression under different conditions and the ability to accurately predict operons are important steps towards understanding the transcriptional regulation, function and pathogenesis.
A whole genome Agilent microarray was developed for the highly virulent, community-acquired MSSA476.During standard growth in a defined medium, we were able to determine four basic gene expression patterns of S. aureus for both virulence and non-virulence genes. In addition, we predicted operon structures by calculating Pearson correlation coefficients of the transcriptional profiles for all adjacent probes over all time points and replicas.
In this study, we have set a basis for the knowledge on gene expression of MSSA476 during growth. Moreover, the correlation of time-dependent transcriptional profiles of adjacent probes seems to be a promising approach to predict operon structures.

Overall design

Five separate cultures of S. aureus mssa476 were grown. Of each replicate culture, samples were taken at 1, 2, 3, 4, 5, 6 and 9 h post inoculation (p.i.). In total, this amounts to 35 samples (7 time points in 5 replicates).

Contributor(s)

Smits N, de Leeuw WC, Fluit AC, Pronk TE, Boel E, Breit TM

Citation(s)

20150412

Submission date

Jun 08, 2009

Last update date

Mar 21, 2012

Contact name

Tessa E Pronk

E-mail(s)

tessa.pronk@rivm.nl