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| Status |
Public on Apr 01, 2011 |
| Title |
Expression profiling MOLT-4 treated with MABL |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of MOLT-4 cells at various time points up to 6 hours following treatment with mouse anti-CD47 antibody (MABL) and goat anti-mouse IgG (GAM) as the crosslinker of MABL. MABL induces apoptosis in CD47-positive MOLT-4 cells. Cell death signals via CD47 ligation were analyzed by using Affymetrix Human Genome U133A microarray.
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| Overall design |
MOLT-4 cell line was obtained from the American Type Culture Collection (ATCC) and grown in RPMI1640 medium (SIGMA) with 10% FBS(JBS). It was maintained at 37 degree C and 5% CO2. Cells were washed with PBS(-) followed by treatment with 10 ug/mL MABL (anti-CD47 antibody) for 0, 0.5, 1, 3 and 6 h. Total RNA was isolated and converted to cDNA using a Superscript Double Stranded cDNA synthesis kit (Invitrogen). Fragmented cDNA was hybridized to Affymetrix Human Genome U133A array and gene expression signals were analyzed. Probes showing >2 or <0.5 fold-change for both replicates compared with the control (geometric mean of 0h replicate signals) were selected at any time.
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| Contributor(s) |
Mizuno H, Kinoshita Y, Fukushima N |
| Citation(s) |
21401803 |
| |
| Submission date |
Jun 08, 2009 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Naoshi Fukushima |
| Organization name |
Chugai pharmaceutical co., ltd.
|
| Department |
Fuji Gotemba labs.
|
| Street address |
Komakado 1-135
|
| City |
Gotemba |
| State/province |
Shizuoka |
| ZIP/Postal code |
412-8513 |
| Country |
Japan |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA116109 |
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