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| Status |
Public on Jan 19, 2021 |
| Title |
Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b |
| Organism |
Capra hircus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Effects were investigated to improve the nuclear reprogramming efficiency by modulating the epigenetic modifications. Long non-coding RNAs (lncRNAs) are involved in shaping chromosome conformation and regulation of preimplantation development. However, the role of lncRNA during nuclear reprogramming remains largely unknown. In the present study, we performed RNA sequencing and identified 687 differentially expressed lncRNAs as candidate key molecules involved in nuclear reprogramming in goat.
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| Overall design |
We performed RNA-seq to identify differencially expressed lncRNAs during embryonic genome activation in goat SCNT embryos, and performed RNA-seq to assess the effect of lnc_3712 knockdown on SCNT reprogramming in goat
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| Contributor(s) |
Deng M, Wan Y, Chen B, Wang F |
| Citation(s) |
33738138 |
| |
| Submission date |
Jan 18, 2021 |
| Last update date |
Mar 22, 2021 |
| Contact name |
Mingtian Deng |
| E-mail(s) |
mtdeng@njau.edu.cn
|
| Organization name |
Nanjing Agricultural University
|
| Department |
College of Animal Science and Technology
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| Lab |
Jiangsu Livestock Embryo Engineering Laboratory
|
| Street address |
Weigang No.1
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210095 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA693046 |
| SRA |
SRP302258 |
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