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Series GSE165219 Query DataSets for GSE165219
Status Public on Jan 22, 2021
Title RIP1 perturbation induces chondrocyte necroptosis and promotes osteoarthritis pathogenesis
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Receptor-interacting protein kinase 1 (RIP1)-mediated necroptosis plays a vital role in various diseases, but the involvement of RIP1 and its functional mechanism in osteoarthritis pathogenesis remains largely unknown. To identify molecular targets of RIP1 in chondrocytes, RNA sequencing was performed in chondrocytes treated with adenovirus expressing RIP1 or vector control. We found that 9857 genes were differentially expressed in chondrocytes after RIP1 overexpression. GO analysis indicated that DNA replication, chromosome segregation and regulation of cell cycle process were upregulated, while terms including cartilage development, skeletal system development, extracellular matrix organization, skeletal system morphogenesis, chondrocyte differentiation, collagen fibril organization and limb development were downregulated. Pathway analysis revealed that IL-17 signaling pathway, cell cycle, DNA replication, proteasome, TNF signaling pathway, cellular senescence and p53 signaling pathway were significantly upregulated by RIP1, meanwhile, ECM-receptor interaction, other glycan degradation and glycosaminoglycan degradation were downregulated. These results underscore the importance of RIP1 in OA by perturbing a series of essential events during disease progression such like cell cycle regulation, chondrocyte differentiation, inflammation and ECM remodeling.
 
Overall design chondrocytes treated with adenovirus expressing RIP1 or vector control.

**Please note that both raw and processed data have been replaced on Mar 22, 2021 by the submitter.
 
Contributor(s) Cheng J, Duan X, Fu X, Jiang Y, Yang P, Cao C, Li Q, Zhang J, Hu X, Zhang X, Ao Y
Citation(s) 33937236
Submission date Jan 21, 2021
Last update date Sep 15, 2021
Contact name cheng jin
E-mail(s) houpengwei@novelbio.com
Organization name Peking University Third Hospital
Street address 49 Huayuan Bei Lu
City beijing
ZIP/Postal code 100000
Country China
 
Platforms (1)
GPL24688 HiSeq X Ten (Rattus norvegicus)
Samples (6)
GSM5028445 CTL-1
GSM5028446 CTL-2
GSM5028447 CTL-3
Relations
BioProject PRJNA693686
SRA SRP302653

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Supplementary file Size Download File type/resource
GSE165219_Allcounts.txt.gz 270.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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