|
| Status |
Public on Jan 27, 2021 |
| Title |
Single cell transcriptomics of primate sensory neurons identifies cell types associated with chronic pain [SmartSeq2] |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Distinct types of dorsal root ganglion sensory neurons may have unique contributions to chronic pain. Identification of primate sensory neuron types is critical for understanding the cellular origin and heritability of chronic pain. However, molecular insights into the primate sensory neurons are missing. Here we classify non-human primate dorsal root ganglion sensory neurons based on their transcriptome and map human pain heritability to neuronal types. First, we identified cell correlates between two major datasets for mouse sensory neuron types. Machine learning exposes an overall cross-species conservation of somatosensory neurons between primate and mouse, although with differences at individual gene level, highlighting the importance of primate data for clinical translation. We map genomic loci associated with chronic pain in human onto primate sensory neuron types to identify the cellular origin of chronic pain. Genome-wide associations for chronic pain converge on two different neuronal types distributed between pain disorders that display different genetic susceptibilities, suggesting both unique and shared mechanisms between different pain conditions.
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| Overall design |
Single cell transcriptomic study of rhesus macaque sensory RDG neurons. This dataset includes 2688 cells using the SmartSeq2 protocol.
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| Contributor(s) |
Usoskin D, Kupari J, Lou D, Parisien M, Hu Y, Fatt M, Lönnerberg P, Linnarsson S, Spångberg M, Eriksson B, Kharchenko P, Nikolaos B, Loré K, Khoury S, Diatchenko L, Ernfors P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 26, 2021 |
| Last update date |
Jan 28, 2021 |
| Contact name |
Sten Linnarsson |
| Organization name |
Karolinska Institutet
|
| Department |
Medical Biochemistry and Biophysics
|
| Lab |
Molecular Neurobiology
|
| Street address |
Scheeles väg 1
|
| City |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL19129 |
Illumina HiSeq 2500 (Macaca mulatta) |
|
| Samples (2688)
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| This SubSeries is part of SuperSeries: |
| GSE165569 |
Single cell transcriptomics of primate sensory neurons identifies cell types associated with human chronic pain |
|
| Relations |
| BioProject |
PRJNA694961 |
| SRA |
SRP303348 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE165553_SS2-16-225_expression_for_R.tab.gz |
1.9 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-227_expression_for_R.tab.gz |
3.8 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-259_expression_for_R.tab.gz |
3.0 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-303_expression_for_R.tab.gz |
3.9 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-304_expression_for_R.tab.gz |
4.9 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-305_expression_for_R_from_comb_plate.tab.gz |
2.7 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-414_expression_for_R_from_comb_plate.tab.gz |
2.7 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-415_expression_for_R_from_comb_plate.tab.gz |
4.1 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2-16-416_expression_for_R_from_comb_plate.tab.gz |
1.1 Mb |
(ftp)(http) |
TAB |
| GSE165553_SS2_SmartSeq2_all_metadata.tab.gz |
25.0 Kb |
(ftp)(http) |
TAB |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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