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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 28, 2021 |
| Title |
10X genomics single cell RNA sequencing of tumor sections from tumor bearing KrasG12D/+Lkb1fl/fl(KL) mice after Ad-cre injection for 10 weeks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Tumor-infiltrating cells comprise many celltypes, including monocytes, macrophages, dendritic cells, neutrophils, stromal cells and epithelial cells, and have emerged as key regulators of cancer growth. These cells can diversify into a spectrum of states, which may promote or limit tumor outgrowth, but remain poorly understood. Here, we used single-cell RNA sequencing to map cell heterogeneity in non-small cell lung cancer mouse model. We uncovered 11 cell types in tumor sections, most of which were reproducibly found across tumor bearing mice. we obtained a series of genes which expression levels were very low, such as cytokines and chemokines. It is very helpful for us to understand the signal cascade and ligands-receptors interaction between different cell types.
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| Overall design |
Single cell RNA sequencing (scRNAseq) of RBC-depleted cells from tumor sections of 2 tumor-bearing KL mice.
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| Contributor(s) |
Wang P, Zhong B |
| Citation(s) |
34369094 |
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| Submission date |
Jan 27, 2021 |
| Last update date |
Oct 19, 2021 |
| Contact name |
鹏 - 王 |
| E-mail(s) |
wangp00678@163.com, wangp94@whu.edu.cn
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| Phone |
+8613071211141
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| Organization name |
武汉大学
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| Street address |
169 Donghu nan road,Wucang District
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| City |
Wuhan |
| State/province |
湖北省 |
| ZIP/Postal code |
430071 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA695259 |
| SRA |
SRP303490 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE165641_RAW.tar |
178.6 Mb |
(http)(custom) |
TAR (of TAR) |
| GSE165641_processed_normalized_matrix_data.Rdata.gz |
39.6 Mb |
(ftp)(http) |
RDATA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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