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Series GSE165641 Query DataSets for GSE165641
Status Public on Jan 28, 2021
Title 10X genomics single cell RNA sequencing of tumor sections from tumor bearing KrasG12D/+Lkb1fl/fl(KL) mice after Ad-cre injection for 10 weeks
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tumor-infiltrating cells comprise many celltypes, including monocytes, macrophages, dendritic cells, neutrophils, stromal cells and epithelial cells, and have emerged as key regulators of cancer growth. These cells can diversify into a spectrum of states, which may promote or limit tumor outgrowth, but remain poorly understood. Here, we used single-cell RNA sequencing to map cell heterogeneity in non-small cell lung cancer mouse model. We uncovered 11 cell types in tumor sections, most of which were reproducibly found across tumor bearing mice. we obtained a series of genes which expression levels were very low, such as cytokines and chemokines. It is very helpful for us to understand the signal cascade and ligands-receptors interaction between different cell types.
 
Overall design Single cell RNA sequencing (scRNAseq) of RBC-depleted cells from tumor sections of 2 tumor-bearing KL mice.
 
Contributor(s) Wang P, Zhong B
Citation(s) 34369094
Submission date Jan 27, 2021
Last update date Oct 19, 2021
Contact name 鹏 - 王
E-mail(s) wangp00678@163.com, 2017202040038@whu.edu.cn
Phone +8613071211141
Organization name 武汉大学
Street address 169 Donghu nan road,Wucang District
City Wuhan
State/province 湖北省
ZIP/Postal code 430071
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (2)
GSM5047302 KL rep1
GSM5047303 KL rep2
Relations
BioProject PRJNA695259
SRA SRP303490

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165641_RAW.tar 178.6 Mb (http)(custom) TAR (of TAR)
GSE165641_processed_normalized_matrix_data.Rdata.gz 39.6 Mb (ftp)(http) RDATA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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