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| Status |
Public on Apr 13, 2022 |
| Title |
Microarray timecourse profiling of gene expression changes in primary human monocytes and B-cells cultured at high density to investigate FcgR2b associated gene expression changes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Whole-genome microarray gene expression profiling of primary human monocytes and B-cells cultured at high density. Monocytes and B-cells were isolated from PMBCs of 2 healthy donors and cultured at 1x10^7 cells/mL (high density). Samples were harvested for microarray gene expresssion profiling at 0, 2, 10 or 24hrs (monocytes) or 0 or 24hrs (B-cells). Protein expression of FcgR2b is higher on monocytes in high density conditions compared to low density (1x10^6 cells/mL) conditions where expression is negligble. This study provides information on genome-wide gene expression changes that occur in high density culture in order to study associations with FcgR2b expression. B-cells cultured at high density and low density show similar levels of moderate FcgR2b expression. B cells at 0hr and after 24hr of high density culture are included in this dataset as a comparators.
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| Overall design |
Microarray timecourse profiling of primary human monocytes and B-cells cultured at high density for 24hr.
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| Contributor(s) |
Smith RC, Thirdborough SM, Hussain K, Cragg MS |
| Citation(s) |
35392965 |
| |
| Submission date |
Jan 27, 2021 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Rosanna C G Smith |
| E-mail(s) |
r.c.smith@soton.ac.uk
|
| Organization name |
University of Southampton
|
| Department |
Cancer Sciences Unit, Faculty of Medicine
|
| Lab |
Antibody and Vaccine Group
|
| Street address |
Centre for Cancer Immunology, University Hospital Southampton (MP 127), Tremona Road
|
| City |
Southampton |
| State/province |
Hampshire |
| ZIP/Postal code |
SO16 6YD |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL13938 |
Illumina HumanHT-12 WG-DASL V4.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA695262 |
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