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| Status |
Public on Sep 17, 2022 |
| Title |
Enterococci enhance Clostridioides difficile pathogenesis |
| Organisms |
Enterococcus faecalis; Clostridioides difficile |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Clostridioides difficile is one of the most common nosocomial pathogens and a global public health threat. Upon colonization of the gastrointestinal tract, C. difficile is exposed to a rapidly changing polymicrobial environment and a dynamic metabolic milieu. Despite the link between the gut microbiota and susceptibility to C. difficile, the impact of synergistic interactions between the microbiota and pathogens on the outcome of infection is largely unknown. Here, we show that microbial cooperation between C. difficile and Enterococcus has a profound impact on the growth, metabolism, and pathogenesis of C. difficile.. Through a process of nutrient restriction and metabolite cross-feeding, E. faecalis shapes the metabolic environment in the gut to enhance C. difficile fitness and increase toxin production. These findings demonstrate that members of the microbiota, such as Enterococcus, have a previously unappreciated impact on C. difficile behavior and virulence.
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| |
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| Overall design |
RNA-sequencing of Clostridioides difficile CD196 and Enterococcus faecalis OG1RF in monoculture and in coculture with three biological replicates per sample
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| |
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| Contributor(s) |
Zackular JP, Smith AB, Di C |
| Citation(s) |
36385534 |
| |
| Submission date |
Jan 28, 2021 |
| Last update date |
Nov 25, 2022 |
| Contact name |
Joseph Zackular |
| E-mail(s) |
Zackular@upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL22510 |
Illumina HiSeq 2500 (Clostridioides difficile) |
| GPL23172 |
Illumina HiSeq 2500 (Enterococcus faecalis) |
| GPL29669 |
Illumina HiSeq 2500 (Clostridioides difficile; Enterococcus faecalis) |
|
| Samples (9)
|
| GSM5049467 |
Clostridioides difficile monoculture rep1 |
| GSM5049468 |
Clostridioides difficile monoculture rep2 |
| GSM5049469 |
Clostridioides difficile monoculture rep3 |
|
| Relations |
| BioProject |
PRJNA695585 |
| SRA |
SRP303739 |
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