|
| Status |
Public on Feb 16, 2022 |
| Title |
Genome-wide acetylation of histone 3 at lysine residuce (H3K27ac) deposition in human iPS cell-derived cardiomyocytes (hiPSC-CMs) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To determine the role of estrogen-related receptor gamma (ERRg) in cardiac myocyte enhancers, we performed ChIP-seq studies with hiPSC-CMs using antibody directed against acetylated histone 3 at lysine residue 27 (H3K27ac), a known active enhancer mark. We found that knocking out ERRg downregulated H3K27ac depositions on several adult cardiac contractile protein and mitochondrial oxidative metabolic genes. These results establish significant roles of ERR to activate cardiac enhancer and promoter activities.
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| |
|
| Overall design |
With ChIP-seq, examination of H3K27ac deposition on the human genome in wild type hiPSC-CMs and ERRg KO hiPSC-CMs.
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| |
|
| Contributor(s) |
Sakamoto T, Batmanov K, Kelly DP |
| Citation(s) |
35418170 |
| |
| Submission date |
Feb 02, 2021 |
| Last update date |
May 05, 2022 |
| Contact name |
Tomoya Sakamoto |
| Organization name |
University of Pennsylvania
|
| Department |
Cardiovascular Institute
|
| Lab |
Daniel Kelly lab
|
| Street address |
Smilow Center for Translational Research 11th FL (Room 11-172) 3400 Civic Center Blvd Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE166064 |
The Nuclear Receptor ERR Cooperates with the Cardiogenic Factor GATA4 to Orchestrate Transcriptional Control of Cardiac Maturation |
|
| Relations |
| BioProject |
PRJNA698779 |
| SRA |
SRP304239 |
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