|
| Status |
Public on Jul 10, 2009 |
| Title |
Tumor T6 Sectors |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
The following CGH experiments were conducted on four sectors (S1-S4) from a single primary ductal carcinoma tumor (T6) using the Sector-Ploidy-Profiling (SPP) Approach. SPP involves macro-dissecting the tumor, flow-sorting nuclei by differences in total genomic DNA content and profiling the genome of the tumor subpopulations.
|
| |
|
| Overall design |
The genomic DNA from each tumor subpopulation was labeled with Cy5 and hybridized to an 85K Bgl2 ROMA Microarray. A normal reference male fibroblast was labeled with Cy3 as a control. Samples were dye-swapped and the experiments were conducted in color-reversal. The value data repesents a log ratio of the mean of two dye-swapped samples
|
| |
|
| Contributor(s) |
Navin NE |
| Citation(s) |
19846608, 19903760 |
| |
| Submission date |
Jun 14, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Nicholas Navin |
| Organization name |
Cold Spring Harbor Laboratory
|
| Lab |
Wigler Lab
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8581 |
85KV4 ROMA Custom Human Microarray (Format 28) |
|
| Samples (5)
|
|
| This SubSeries is part of SuperSeries: |
| GSE16672 |
All Tumor Sector Experiments (T1-T20) |
|
| Relations |
| BioProject |
PRJNA122921 |
Less...
More...