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| Status |
Public on Mar 08, 2021 |
| Title |
Assessing the plasticity of the senescent phenotype |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The senescent phenotype was evaluated to observe potential changes over time and on differing culture substrates.
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| Overall design |
While numerous studies have characterized the in vitro-derived senescent phenotype of cells in culture, all of these studies observed cells within a short time-frame and while cultured on tissue culture plastic. We investigated whether the senescent phenotype changes over time or is affected by the substrate/context in which the cells are cultured. To this end, we worked with primary small airway epithelial cells where senescence was induced while cells were cultured on Matrigel. In addition, we also cultured senescent cells for a short, 5 days, and long period, 12 days, of time to observe if the phenotype of these cells is altered.
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| Contributor(s) |
DePianto DJ, Morshead KB, Vander Heiden JA, Arron JR |
| Citation(s) |
33705361 |
| |
| Submission date |
Feb 02, 2021 |
| Last update date |
May 25, 2021 |
| Contact name |
Jason Anthony Vander Heiden |
| E-mail(s) |
vandej27@gene.com
|
| Organization name |
Genentech
|
| Department |
Biological Research AI Development
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE166059 |
Molecular mapping of interstitial lung disease reveals a phenotypically distinct senescent basal epithelial cell population |
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| Relations |
| BioProject |
PRJNA699010 |
| SRA |
SRP304379 |
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