 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 04, 2021 |
| Title |
Role of the transcriptional regulator SP140 in resistance to bacterial infection via repression of type I interferons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Type I interferons (IFNs) are essential for anti-viral immunity, but often impair protective immune responses during bacterial infections. An important question is how type I IFNs are strongly induced during viral infections, and yet are appropriately restrained during bacterial infections. The Super susceptibility to tuberculosis 1 (Sst1) locus in mice confers resistance to diverse bacterial infections. Here we provide evidence that Sp140 is a gene encoded within the Sst1 locus that represses type I IFN transcription during bacterial infections. We generated Sp140–/– mice and find they are susceptible to infection by Legionella pneumophila and Mycobacterium tuberculosis. Susceptibility of Sp140–/– mice to bacterial infection was rescued by crosses to mice lacking the type I IFN receptor (Ifnar–/–). Our results implicate Sp140 as an important repressor of type I IFNs that is essential for resistance to bacterial infections.
|
| |
|
| Overall design |
Lung mRNA profiles of B6, B6.Sst1S, Sp140-/-, Sp110-/- mice infected with M. tuberculosis. Day 25 post-infection.
|
| |
|
| Contributor(s) |
Ji DX, Witt KC, Kotov DI, Margolis SR, Louie A, Chevée V, Chen KJ, Dhaliwal HS, Lee AY, Zamboni DS, Kramnik I, Portnoy DA, Darwin KH, Vance RE |
| Citation(s) |
34151776 |
| |
| Submission date |
Feb 03, 2021 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Daisy Ji |
| E-mail(s) |
daisyji@hsph.harvard.edu
|
| Organization name |
Harvard University School of Public Health
|
| Street address |
665 Huntington Avenue, Building 1, Room 811
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA699285 |
| SRA |
SRP304574 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE166114_RAW.tar |
14.0 Mb |
(http)(custom) |
TAR (of TSV) |
| GSE166114_raw_counts.txt.gz |
375.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...