GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE166114 Query DataSets for GSE166114
Status Public on Feb 04, 2021
Title Role of the transcriptional regulator SP140 in resistance to bacterial infection via repression of type I interferons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Type I interferons (IFNs) are essential for anti-viral immunity, but often impair protective immune responses during bacterial infections. An important question is how type I IFNs are strongly induced during viral infections, and yet are appropriately restrained during bacterial infections. The Super susceptibility to tuberculosis 1 (Sst1) locus in mice confers resistance to diverse bacterial infections. Here we provide evidence that Sp140 is a gene encoded within the Sst1 locus that represses type I IFN transcription during bacterial infections. We generated Sp140–/– mice and find they are susceptible to infection by Legionella pneumophila and Mycobacterium tuberculosis. Susceptibility of Sp140–/– mice to bacterial infection was rescued by crosses to mice lacking the type I IFN receptor (Ifnar–/–). Our results implicate Sp140 as an important repressor of type I IFNs that is essential for resistance to bacterial infections.
Overall design Lung mRNA profiles of B6, B6.Sst1S, Sp140-/-, Sp110-/- mice infected with M. tuberculosis. Day 25 post-infection.
Contributor(s) Ji DX, Witt KC, Kotov DI, Margolis SR, Louie A, Chevée V, Chen KJ, Dhaliwal HS, Lee AY, Zamboni DS, Kramnik I, Portnoy DA, Darwin KH, Vance RE
Citation(s) 34151776
Submission date Feb 03, 2021
Last update date Jul 08, 2021
Contact name Daisy Ji
Organization name Harvard University School of Public Health
Street address 665 Huntington Avenue, Building 1, Room 811
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM5061928 Lung, B6 rep1
GSM5061929 Lung, B6 rep2
GSM5061930 Lung, B6.Sst1S rep1
BioProject PRJNA699285
SRA SRP304574

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166114_RAW.tar 14.0 Mb (http)(custom) TAR (of TSV)
GSE166114_raw_counts.txt.gz 375.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap