|
| Status |
Public on Mar 17, 2021 |
| Title |
Genome-wide binding profiles of KDM5 in Drosophila GMCs and immature neurons |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Mutations in the lysine demethylase 5 (KDM5) family of transcriptional regulators are associated with intellectual disability, yet little is known regarding the spatiotemporal requirements or neurodevelopmental contributions of KDM5 proteins. Utilizing the mushroom body (MB), a major learning and memory center within the Drosophila brain, we demonstrate that KDM5 is specifically required within ganglion mother cells and immature neurons for proper neurodevelopment. Within this cellular subpopulation, we identify a core network of KDM5-regulated and -bound genes that are critical modulators of neurodevelopment. Significantly, we find that a majority of these genes are direct targets of Prospero (Pros), a transcription factor with well-established roles in neurodevelopment in other neuronal contexts. We demonstrate that Pros is essential for MB development and show that KDM5 binds to pros and regulates its expression. We therefore provide evidence for a KDM5-Pros axis that orchestrates a transcriptional program critical for proper neurodevelopment.
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| Overall design |
9 samples with 6 biological replicates for R71C09 > dam:kdm5 with tubulin-Gal80[ts] and 3 biological replicates for R71C09 > dam with tubulin-Gal80[ts].
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| Contributor(s) |
Hatch HA, Belalcazar HM, Marshall OJ, Secombe J |
| Citation(s) |
33729157, 37800333 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 NS110278 |
Transcriptional regulation, neuronal development, and function of the mushroom body in a Drosophila model of intellectual disability |
ALBERT EINSTEIN COLLEGE OF MEDICINE |
Hayden Alexander Moses Hatch |
| T32 GM007288 |
Medical Scientist Training Program |
ALBERT EINSTEIN COLLEGE OF MEDICINE |
Myles H. Akabas |
| R01 GM112783 |
Distinct modes of gene expression by KDM5 |
ALBERT EINSTEIN COLLEGE OF MEDICINE |
Julie Secombe |
| P30 CA013330 |
Core Support for Cancer Center |
ALBERT EINSTEIN COLLEGE OF MEDICINE |
EDWARD CHU |
| S10 OD023591 |
Advanced Confocal Microscope in a multi-user facility |
ALBERT EINSTEIN COLLEGE OF MEDICINE, INC. |
Nicholas E Baker |
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| |
| Submission date |
Feb 03, 2021 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Hayden A. M. Hatch |
| E-mail(s) |
hayden.hatch@einsteinmed.org
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Dominick P. Purpura Department of Neuroscience; Department of Genetics
|
| Lab |
Laboratory of Julie Secombe, Ph.D.
|
| Street address |
1300 Morris Park Avenue, Ullmann 809
|
| City |
Bronx |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL26560 |
BGISEQ-500 (Drosophila melanogaster) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA699290 |
| SRA |
SRP304586 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE166116_damkdm5-vs-Dam_1.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5-vs-Dam_2.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5-vs-Dam_3.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5-vs-Dam_4.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5-vs-Dam_5.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5-vs-Dam_6.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5-vs-Dam_average.gatc.bedgraph.gz |
5.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE166116_damkdm5genes.xlsx |
213.4 Kb |
(ftp)(http) |
XLSX |
| GSE166116_kdm5-vs-Dam.gatc.average-FDR0.01.peaks.gff.gz |
50.1 Kb |
(ftp)(http) |
GFF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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