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Series GSE166137 Query DataSets for GSE166137
Status Public on Feb 05, 2021
Title The involvement of Mycobacterium type III-A CRISPR-Cas system in oxidative stress and intracellular fitness
Organism Mycobacterium tuberculosis variant bovis
Experiment type Expression profiling by high throughput sequencing
Summary Previous studies suggest that type I and type II CRISPR-Cas systems were employed to evade host immunity by targeting interference of bacteria’s own genes. Although Mycobacterium bovis (M. bovis) and Mycobacterium tuberculosis (M. tuberculosis) possess integrated type III-A CRISPR-Cas system, its role in mycobacteria remains obscure. Here, we observed that seven cas genes (csm2~6, cas10, cas6) were significantly upregulated under oxidative stress treatment. To explore the function of type III-A CRSIRP-Cas system, Total CRISPR system (TCR) mutant strain was generated in M. bovis Bacille Calmette-Guérin (BCG). Deletion of TCR results in increased sensitivity in response to H2O2 and reduced cell envelope integrity. By using RNA-Seq, 590 differential expression genes were found in mutant for TCR, of which 220 genes were upregulated and 370 genes were downregulated, indicating an important role of TCR in controlling gene expression in mycobacteria. Consistently, disrupting TCR results in poor intracellular survival in vivo and in vitro. Moreover, we showed for the first time that TCR contributed to the regulation of regulatory T cell population, supporting a role of TCR in modulating host immunity. These observations demonstrate that type III-A CRISPR-Cas system as an important factor for intracellular survival and host immunoregulation in mycobacteria, may be a potential target for therapy.
Overall design For RNA-seq: Examination of 2 strains (Wild type VS ΔTCR).
Contributor(s) Feng S
Citation(s) 34956138
Submission date Feb 04, 2021
Last update date Jan 10, 2022
Contact name siyuan feng
Organization name Sun Yat-Sen University
Street address 74 zhongshan 2nd road
City Guang zhou
ZIP/Postal code 510080
Country China
Platforms (1)
GPL29695 Illumina HiSeq 2000 (Mycobacterium tuberculosis variant bovis)
Samples (6)
GSM5062170 WT-1: wild type BCG
GSM5062171 WT-2: wild type BCG
GSM5062172 WT-3: wild type BCG
BioProject PRJNA699439
SRA SRP304681

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Supplementary file Size Download File type/resource
GSE166137_Table_S2.csv.gz 13.8 Kb (ftp)(http) CSV
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