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| Status |
Public on Feb 05, 2021 |
| Title |
The involvement of Mycobacterium type III-A CRISPR-Cas system in oxidative stress and intracellular fitness |
| Organism |
Mycobacterium tuberculosis variant bovis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous studies suggest that type I and type II CRISPR-Cas systems were employed to evade host immunity by targeting interference of bacteria’s own genes. Although Mycobacterium bovis (M. bovis) and Mycobacterium tuberculosis (M. tuberculosis) possess integrated type III-A CRISPR-Cas system, its role in mycobacteria remains obscure. Here, we observed that seven cas genes (csm2~6, cas10, cas6) were significantly upregulated under oxidative stress treatment. To explore the function of type III-A CRSIRP-Cas system, Total CRISPR system (TCR) mutant strain was generated in M. bovis Bacille Calmette-Guérin (BCG). Deletion of TCR results in increased sensitivity in response to H2O2 and reduced cell envelope integrity. By using RNA-Seq, 590 differential expression genes were found in mutant for TCR, of which 220 genes were upregulated and 370 genes were downregulated, indicating an important role of TCR in controlling gene expression in mycobacteria. Consistently, disrupting TCR results in poor intracellular survival in vivo and in vitro. Moreover, we showed for the first time that TCR contributed to the regulation of regulatory T cell population, supporting a role of TCR in modulating host immunity. These observations demonstrate that type III-A CRISPR-Cas system as an important factor for intracellular survival and host immunoregulation in mycobacteria, may be a potential target for therapy.
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| Overall design |
For RNA-seq: Examination of 2 strains (Wild type VS ΔTCR).
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| Contributor(s) |
Feng S |
| Citation(s) |
34956138 |
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| Submission date |
Feb 04, 2021 |
| Last update date |
Jan 10, 2022 |
| Contact name |
siyuan feng |
| E-mail(s) |
fsy3224502@gmail.com
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| Organization name |
Sun Yat-Sen University
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| Street address |
74 zhongshan 2nd road
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| City |
Guang zhou |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
| GPL29695 |
Illumina HiSeq 2000 (Mycobacterium tuberculosis variant bovis) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA699439 |
| SRA |
SRP304681 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE166137_Table_S2.csv.gz |
13.8 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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