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Status |
Public on Jul 26, 2021 |
Title |
Characterization of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Pancreatic polypeptide (PP) cells, which secrete PP encoded by the Ppy gene, is a minor population of endocrine cells in the pancreas. At present, little is known about the characteristics of Ppy-expressing cells. Using a Ppy-Cre driver, here we show that cells of the Ppy lineage contribute to four major types of endocrine cells, including beta cells. Unbiased single-cell analysis with a Ppy-lineage tracer demonstrated that beta cells are composed of seven clusters, which are categorized into two groups; i.e., Ppy-lineage and non-Ppy-lineage beta cells. These subpopulations of beta cells demonstrated distinct characteristics in terms of functionality and gene expression profiles. Ppy-lineage beta cells are less active in glucose-stimulated calcium signaling, are localized at the periphery of islets, and survive for long periods in experimental diabetes models, and sharing various molecular characteristics with PP cells. Our results indicate the unexpected degree of beta-cell heterogeneity defined by Ppy-gene activation, providing valuable insights into the homeostatic regulation of pancreatic islets, and future therapeutic strategies against diabetes.
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Overall design |
scRNA-seq (10× Genomics Chromium Single Cell 3′ Solution v3.1) of islets from 8-week-old (postnatal day 56) healthy male MIP-GFP; Ppy-Cre; Rosa26-tdTomato mice with a mixed background of C57BL/6J and CD-1.
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Contributor(s) |
Fujitani Y |
Citation(s) |
34498099 |
BioProject |
PRJNA694512 |
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Submission date |
Feb 04, 2021 |
Last update date |
Oct 08, 2021 |
Contact name |
Yoshio Fujitani |
E-mail(s) |
fujitani@gunma-u.ac.jp
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Phone |
0272208855
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Organization name |
Institute for Molecular and Cellular Regulation
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Department |
Developmental Biology and Metabolism
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Street address |
3-39-15 Showa-machi,
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City |
Maebashi |
State/province |
Gunma |
ZIP/Postal code |
371-8512 |
Country |
Japan |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (1) |
GSM5065049 |
Pancreatic islets cells from MIP-GFP; Ppy-Cre; Rosa26-tdTomato mouse |
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Relations |
SRA |
SRP303088 |
Supplementary file |
Size |
Download |
File type/resource |
GSE166164_RAW.tar |
19.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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