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Series GSE166164 Query DataSets for GSE166164
Status Public on Jul 26, 2021
Title Characterization of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Pancreatic polypeptide (PP) cells, which secrete PP encoded by the Ppy gene, is a minor population of endocrine cells in the pancreas. At present, little is known about the characteristics of Ppy-expressing cells. Using a Ppy-Cre driver, here we show that cells of the Ppy lineage contribute to four major types of endocrine cells, including beta cells. Unbiased single-cell analysis with a Ppy-lineage tracer demonstrated that beta cells are composed of seven clusters, which are categorized into two groups; i.e., Ppy-lineage and non-Ppy-lineage beta cells. These subpopulations of beta cells demonstrated distinct characteristics in terms of functionality and gene expression profiles. Ppy-lineage beta cells are less active in glucose-stimulated calcium signaling, are localized at the periphery of islets, and survive for long periods in experimental diabetes models, and sharing various molecular characteristics with PP cells. Our results indicate the unexpected degree of beta-cell heterogeneity defined by Ppy-gene activation, providing valuable insights into the homeostatic regulation of pancreatic islets, and future therapeutic strategies against diabetes.
 
Overall design scRNA-seq (10× Genomics Chromium Single Cell 3′ Solution v3.1) of islets from 8-week-old (postnatal day 56) healthy male MIP-GFP; Ppy-Cre; Rosa26-tdTomato mice with a mixed background of C57BL/6J and CD-1.
 
Contributor(s) Fujitani Y
Citation(s) 34498099
BioProject PRJNA694512
Submission date Feb 04, 2021
Last update date Oct 08, 2021
Contact name Yoshio Fujitani
E-mail(s) fujitani@gunma-u.ac.jp
Phone 0272208855
Organization name Institute for Molecular and Cellular Regulation
Department Developmental Biology and Metabolism
Street address 3-39-15 Showa-machi,
City Maebashi
State/province Gunma
ZIP/Postal code 371-8512
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (1)
GSM5065049 Pancreatic islets cells from MIP-GFP; Ppy-Cre; Rosa26-tdTomato mouse
Relations
SRA SRP303088

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166164_RAW.tar 19.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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