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Series GSE166239 Query DataSets for GSE166239
Status Public on Dec 01, 2023
Title Partial epithelial-to-mesenchymal transition (EMT) and T cell-mediated inflammation in hypertensive and diabetic nephropathy
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We comparatively evaluated transcriptomes from renal biopsies obtained from patients with T2DN or HN, main causes of CKD, and control renal tissues (n=6 per group). RNA was extracted from formalin-fixed and paraffin-embedded kidney samples and processed for RNA sequencing. Principal component analysis effectively separated diseased and control tissues. Gene and protein expression profiles revealed that EMT-related markers were upregulated in both diseases. Moreover, activation of CD4 and CD lymphocytes, as well as upregulation of the complement system were also observed in both T2DN and HN. These data, suggesting partially common pathogenetic mechanisms, were largely confirmed by IHC analysis.
Overall design Formalin-fixed and paraffin-embedded kidney biopsies from adult patients were selected from the Norwegian Kidney Biopsy Registry. Specimens from patients with HN, T2DN and normal-appearing control renal biopsies (Ctrl) (n=6 for each group) were assessed. Total RNA was extracted from 10 μm whole kidney sections and processed for RNA sequencing, while immunohistochemistry (IHC) was performed on 3-μm tissue sections.
*** Submitter declares that the raw data is being deposited in the European Genome-phenome Archive (EGA) due to patient privacy concerns.***
Contributor(s) Nordbo OP, Landolt L, Mydel P, Eikrem O, Scherer A, Leh S, Furriol Palmer J, Apeland T, Marti H
Citation(s) 37813528
Submission date Feb 05, 2021
Last update date Dec 18, 2023
Contact name Philipp Strauss
Organization name University of Bergen
Street address Haukeland Universitetssykehus Laboratoriebygget, 7. etg. Heis øst
City Bergen
ZIP/Postal code 5020
Country Norway
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM5066699 Ctrl_1
GSM5066700 Ctrl_2
GSM5066701 Ctrl_3
BioProject PRJNA699758

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166239_Nordbo_et_al_counts.txt.gz 446.8 Kb (ftp)(http) TXT
GSE166239_Nordbo_et_al_counts_logCPMnorm.txt.gz 1.2 Mb (ftp)(http) TXT
Processed data are available on Series record
Raw data not provided for this record

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