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| Status |
Public on Oct 31, 2021 |
| Title |
transcript analysis of strains with or without plasmid containing 40 genes in three different culture conditions |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To investigate the influence of the 40 genes on plasmid, we compared the transcript varies between the engineered strains and the wild-type strain in synthetic medium with different carbon sources (i.e., glucose, galactose and ethanol/glycerol media).
Methods: Total mRNA profiles of the engineered strains and the wild-type strain were generated by deep sequencing, in triplicate, using BGIseq500. The sequence reads that passed quality filters were analyzed.
Results: RNA sequencing results revealed that the transcript patterns were influenced dramatically by the carbon sources and there was no significant difference between the wild-type and engineered strains (that is, any change amounted to less than 10%)
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| Overall design |
Total mRNA profiles of the engineered strains and the wild-type strain which grew to log phase and collected.
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| Contributor(s) |
Cheng L, Dai J |
| Citation missing |
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| |
| Submission date |
Feb 08, 2021 |
| Last update date |
Nov 01, 2021 |
| Contact name |
Li Cheng |
| E-mail(s) |
li.cheng@siat.ac.cn
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| Organization name |
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
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| Department |
Institute of Synthetic Biology
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| Street address |
Xueyuan Boulevard
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| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
| GPL25927 |
BGISEQ-500 (Saccharomyces cerevisiae) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA700522 |
| SRA |
SRP305282 |
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