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| Status |
Public on Jul 10, 2021 |
| Title |
The influence of sex and age on the lens injury response |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The purpose of this study was to investigate/characterize age- and sex-dependent changes in transcriptomic profile in a mouse model of cataract surgery that mimics human cataract surgery. This surgery involves the removal of lens fiber cells (LFC) leaving behind the lens capsule and associated lens epithelial cells (LEC). The lens capsule and associated remnant LECs were harvested either following cataract surgery (0 hour) or 24 hours later. Fiber cells were collected at the time of surgery, equivalent to 0 hour, and analyzed separately from epithelial cells.
For samples from LEC, each biological replicate is a pool of 5 lens capsules from 5 independent mice.
For samples from LFC, each biological replicate is a pool of 2 fiber cell masses from two independent mice.
Mice were either 24 months old (Aged), or 3 months old (Young).
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| Overall design |
A total of 24 biological replicates were sequenced, with 2 male and 2 female biological replicates per experimental condition.
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| Contributor(s) |
Faranda AP, Shihan MH, Wang Y, Duncan MK |
| Citation(s) |
34119483 |
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| Submission date |
Feb 11, 2021 |
| Last update date |
Apr 26, 2024 |
| Contact name |
Melinda K. Duncan |
| E-mail(s) |
duncanm@udel.edu
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| Phone |
3028310533
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| Organization name |
University of Delaware
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| Department |
Biological Sciences
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| Lab |
Vertebrate Development
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| Street address |
327 Wolf Hall
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| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19716 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (24)
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| GSM5076522 |
Lens Epithelium from Male Aged Mice, 0 Hours post surgery, rep 1 |
| GSM5076523 |
Lens Epithelium from Male Aged Mice, 0 Hours post surgery, rep 2 |
| GSM5076524 |
Lens Epithelium from Female Aged Mice, 0 Hours post surgery, rep 1 |
| GSM5076525 |
Lens Epithelium from Female Aged Mice, 0 Hours post surgery, rep 2 |
| GSM5076526 |
Lens Epithelium from Male Aged Mice, 24 Hours post surgery, rep 1 |
| GSM5076527 |
Lens Epithelium from Male Aged Mice, 24 Hours post surgery, rep 2 |
| GSM5076528 |
Lens Epithelium from Female Aged Mice, 24 Hours post surgery, rep 1 |
| GSM5076529 |
Lens Epithelium from Female Aged Mice, 24 Hours post surgery, rep 2 |
| GSM5076530 |
Lens Fibers from Male Aged Mice, 0 Hours post surgery, rep 1 |
| GSM5076531 |
Lens Fibers from Male Aged Mice, 0 Hours post surgery, rep 2 |
| GSM5076532 |
Lens Fibers from Female Aged Mice, 0 Hours post surgery, rep 1 |
| GSM5076533 |
Lens Fibers from Female Aged Mice, 0 Hours post surgery, rep 2 |
| GSM5076534 |
Lens Epithelium from Male Young Mice, 0 Hours post surgery, rep 1 |
| GSM5076535 |
Lens Epithelium from Male Young Mice, 0 Hours post surgery, rep 2 |
| GSM5076536 |
Lens Epithelium from Female Young Mice, 0 Hours post surgery, rep 1 |
| GSM5076537 |
Lens Epithelium from Female Young Mice, 0 Hours post surgery, rep 2 |
| GSM5076538 |
Lens Epithelium from Male Young Mice, 24 Hours post surgery, rep 1 |
| GSM5076539 |
Lens Epithelium from Male Young Mice, 24 Hours post surgery, rep 2 |
| GSM5076540 |
Lens Epithelium from Female Young Mice, 24 Hours post surgery, rep 1 |
| GSM5076541 |
Lens Epithelium from Female Young Mice, 24 Hours post surgery, rep 2 |
| GSM5076542 |
Lens Fibers from Male Young Mice, 0 Hours post surgery, rep 1 |
| GSM5076543 |
Lens Fibers from Male Young Mice, 0 Hours post surgery, rep 2 |
| GSM5076544 |
Lens Fibers from Female Young Mice, 0 Hours post surgery, rep 1 |
| GSM5076545 |
Lens Fibers from Female Young Mice, 0 Hours post surgery, rep 2 |
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| Relations |
| BioProject |
PRJNA701425 |
| SRA |
SRP305864 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE166619_All0H.BySex_FAE0vFYE0_ExactTestQVL_Differential_Expression.xlsx |
15.0 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FAE0vMAE0_ExactTestQVL_Differential_Expression.xlsx |
15.0 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FAF0vFAE0_ExactTestQVL_Differential_Expression.xlsx |
16.0 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FAF0vFYF0_ExactTestQVL_Differential_Expression.xlsx |
16.1 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FAF0vMAF0_ExactTestQVL_Differential_Expression.xlsx |
15.8 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FYE0vMYE0_ExactTestQVL_Differential_Expression.xlsx |
14.1 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FYF0vFYE0_ExactTestQVL_Differential_Expression.xlsx |
16.7 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_FYF0vMYF0_ExactTestQVL_Differential_Expression.xlsx |
15.5 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_MAE0vMYE0_ExactTestQVL_Differential_Expression.xlsx |
15.6 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_MAF0vMAE0_ExactTestQVL_Differential_Expression.xlsx |
17.5 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_MAF0vMYF0_ExactTestQVL_Differential_Expression.xlsx |
15.9 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H.BySex_MYF0vMYE0_ExactTestQVL_Differential_Expression.xlsx |
17.0 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H_AE0vYE0_ExactTestQVL_Differential_Expression.xlsx |
13.0 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H_AF0vAE0_ExactTestQVL_Differential_Expression.xlsx |
15.2 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H_AF0vYF0_ExactTestQVL_Differential_Expression.xlsx |
15.8 Mb |
(ftp)(http) |
XLSX |
| GSE166619_All0H_YF0vYE0_ExactTestQVL_Differential_Expression.xlsx |
16.2 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_FAE24vFAE0_ExactTestQVL_Differential_Expression.xlsx |
16.7 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_FAE24vFYE24_ExactTestQVL_Differential_Expression.xlsx |
16.5 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_FAE24vMAE24_ExactTestQVL_Differential_Expression.xlsx |
16.4 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_FYE24vFYE0_ExactTestQVL_Differential_Expression.xlsx |
16.9 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_FYE24vMYE24_ExactTestQVL_Differential_Expression.xlsx |
15.5 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_MAE24vMAE0_ExactTestQVL_Differential_Expression.xlsx |
18.2 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_MAE24vMYE24_ExactTestQVL_Differential_Expression.xlsx |
17.1 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi.BySex_MYE24vMYE0_ExactTestQVL_Differential_Expression.xlsx |
17.4 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi_AE24vAE0_ExactTestQVL_Differential_Expression.xlsx |
15.1 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi_AE24vYE24_ExactTestQVL_Differential_Expression.xlsx |
14.3 Mb |
(ftp)(http) |
XLSX |
| GSE166619_AllEpi_YE24vYE0_ExactTestQVL_Differential_Expression.xlsx |
15.5 Mb |
(ftp)(http) |
XLSX |
| GSE166619_RAW.tar |
4.4 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE166619_README.txt |
4.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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